ANTIBIOGRAM OF MICROBES ASSOCIATED WITH ENTERIC INFECTION TARGETING INCIDENCES OF MULTIDRUG RESISTANCE

Main Article Content

SHIVANGI DIXIT
SARIKA GUPTA

Abstract

Background and Objective: The Enterobacteriaceae family includes several genera that are biochemically and genetically related to each other and that cause primary gastrointestinal tract infections. Members of this family are Enterobacter spp., Escherichia spp., Klebsiella spp., and Pseudomonas spp., etc. major causes of opportunistic infection (including Diarrhea, Dysentery, Shigellosis, and Salmonellosis). The multidrug-resistant organisms (MDROs) are a major public health problem, on a global level relate to significant mortality and morbidity. Enterobacteriaceae reduced resistant to penicillin’s, broad-spectrum ESBLs production cephalosporin and monobactams are known as the main cause of both community and nosocomial-acquired infections.

Methods: The samples were collected as raw unprocessed and processed food. Preliminary all the samples were subjected to microbiological analysis for the isolation and identification of microbial species in the test samples. It is followed by the assessment of the resistance index of different antibiotics.

Results: 12 food samples were collected and analyzed microbiologically. A total of 32 bacterial isolates were recovered from different samples. The present data revealed the occurrence of 65.2% Gram-Positive bacteria and 34.7% Gram-Negative bacteria among the screened microbial isolates. The potential Enterobacteriaceae were found to be 99.9% among gram-negative isolates as Klebsiella pneumoniae, Enterobacter aerogenes,        E. coli, Pseudomonas aeruginosa. The bacterial isolates reported maximum resistance to Cefazolin (82.35%) followed by maximum resistance to Ampicillin (47.05%), Lemefloxacin and Cefixime (41.17%), Cefuroxime and Nitrofurantoin (29.41%), Cotrimoxzole and Imipenem (23.5%), Fosfomycin (17.6%), Amoxyclav and Norfloxacin (11.76%), Amikacin, Gentamicin, Cefpotoxime, Piperacillin, Ticarcillin, and Meropenem (5.88%).

Conclusion: The present study investigates and assesses incidences of microbes associated with enteric infection from the raw and processed food and to characterize them on the basis of antibiogram, thereby analysis drug resistance among the population of bacteria.

Keywords:
Enterobacteriaceae, food borne disease, food borne illness, multidrug resistant

Article Details

How to Cite
DIXIT, S., & GUPTA, S. (2019). ANTIBIOGRAM OF MICROBES ASSOCIATED WITH ENTERIC INFECTION TARGETING INCIDENCES OF MULTIDRUG RESISTANCE. Journal of Disease and Global Health, 1233(2), 41–45. Retrieved from http://ikprress.org/index.php/JODAGH/article/view/4827
Section
Original Research Article

References

Jarząb A, Górska-Frączek S, Rybka J, Witkowska D. Enterobacteriaceae infection-diagnosis, antibiotic resistance and prevention. Post epyhigienyimedycynydoswiadczalnej (Online). 2011;65(2):55-72.

Bain R, Cronk R, Hossain R, Bonjour S, Onda K, Wright J, Bartram J. Global assessment of exposure to faecal contamination through drinking water based on a systematic review. Tropical Medicine and International Health. 2014;19(8):917-927.

Baylis C, Uyttendaele M, Joosten H, Davies A. The Enterobacteriaceae and their significance to the food industry. ILSI Europe Report Series. 2011;1-48.

Oliver SP, Boor KJ, Murphy SC, Murinda SE. Food safety hazards associated with consumption of raw milk. Food Borne Pathogens and Disease. 2009;6(7):793-806.

Persijn JP, Vander Slik W. A new method for the determination of γ-glutamyl transferase in serum. Clinical Chemistry and Laboratory Medicine. 1976;14(1-12):421-428.

Costa CA, Trivelato GC, Pinto AM, Bechara EJ. Correlation between plasma 5-aminolevulinic acid concentrations and indicators of oxidative stress in lead-exposed workers. Clinical Chemistry. 1997;43(7):1196-1202.

Centers for Disease Control and Prevention. Guide to confirming an etiology in food borne disease outbreak; 2011.
Available:https://www.cdc.gov/foodsafety/outbreaks/investigating outbreaks/confirming_diagnosis.html

Siegel JD, Rhinehart E, Jackson M, Chiarello L. Guideline for isolation precautions preventing transmission of infectious agents in healthcare settings. 2007;1-204.

Patel G, Bonomo R. Stormy waters ahead: Global emergence of carbapenemases. Frontiers in Microbiology. 2013;4(10):48.

Bush K, Fisher JF. Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from gram-negative bacteria. Annual Review of Microbiology. 2011;65(1):455-478.

Bradford PA, Bratu S, Urban C, Visalli M, Mariano N, Landman D, Quale J. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City. Clinical Infectious Diseases. 2004;39(1):55-60.

Li Y-H, Tian X. Quorum sensing and bacterial social interactions in biofilms. Sensors. 2012;12(4):2519–2538.

Wang HH, Schaffner DW. Antibiotic resistance: How much do we know and where do we go from here? Applied Environmental Microbiology. 2011;77(20):7093-7095.

Boehme S, Werner G, Klare I, Reissbrodt R, Witte W. Occurrence of antibiotic resistant enterobacteria in agricultural foodstuffs. Molecular Nutrition and Food Research. 2004;48(7):522-531.

Johnston CS, Tjonn SL, Swan PD. High-protein, low-fat diets are effective for weight loss and favorably alter biomarkers in healthy adults. The Journal of Nutrition. 2004;134(3): 586-591.

Snyder ML, Herman CL. Sodium Azide as an inhibiting substance for gram- negative bacteria. The Journal of Infectious Diseases. 1940;67(2):113-115.

Cappuccino JG, Sherman N. Microbiology: A laboratory manual. 10th Edition. Pearson Benjamin Cummings. 2013;13(9):566.

Bergey DH, Breed RS. Bergey's manual of determinative bacteriology. 7th Ed. American Journal of Public Health and the Nations Health. 1971;54(3):544.

Wayne PA. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2011;31(1):100-121.

Reddi SGDNL, Kumar R, Balakrishna N, Rao VS. Micro-biological quality of street vended fruit juices in Hyderabad, India and their association between food safety knowledge and practices of fruit juice vendors. International Journal of Current Microbiology and Applied Sciences. 2015;4(1):970–982.

Kechero FK, Baye K, Tefera AT, Tessema TS. Bacteriological quality of commonly consumed fruit juices and vegetable salads sold in some fruit juice houses in Addis Ababa, Ethiopia. Journal of Food Safety. 2019;39(1):1-9.

Amare A, Worku T, Ashagirie B, Adugna M, Getaneh A, Dagnew M. Bacteriological profile, antimicrobial susceptibility patterns of the isolates among street vended foods and hygienic practice of vendors in Gondar town, Northwest Ethiopia: A cross sectional study. BMC Microbiology. 2019;19(120):1-9.

Lee JH, Cho MH, Lee J. 3-Indolylacetonitrile decreases Escherichia coli O157: H7 biofilm formation and Pseudomonas aeruginosa virulence. Environmental Microbiology. 2011;13(1):62-73.

Wang HH, Schaffner DW. Antibiotic resistance: How much do we know and where do we go from here? Applied Environmental Microbiology. 2011;77(20):7093-7095.

Manuzon MY, Hanna SE, Luo H, Yu Z, Harper WJ, Wang HH. Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR. Applied and Environmental Microbiology. 2007;73(5):1676-1677.

Resch M, Nagel V, Hertel C. Antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. International Journal of Food Microbiology. 2008;127(1-2):99-104.

Thanner S, Drissner D, Walsh F. Antimicrobial resistance in agriculture. M Bio. 2016;7(2):1-7.

Ye Q, Wu Q, Zhang S, Zhang J, Yang G, Wang J, Chen M. Characterization of extended-spectrum β-lactamase-producing Enterobacteriaceae from retail food in China. Frontiers in Microbiology. 2018;9(4):1709.