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Demand for growing golden camellias has been increasing as their yellow flowers have been widely used. Tea made by its flowers has been used for healthcare and the importance of using flowers on antioxidant activities, superoxide anions and hydroxyl free radicals scavenging assays has been reported. In addition, flowers are also used to treat sore throat, diarrhea, and cancer prevention. In this study, effects of pre-sowing treatment and storage on seed germination were examined for a golden camellia - Camellia impressinervis. Seeds were collected from planted trees in North Vietnam for experiments. There were two storage treatments as clean seeds were stored in a refrigerator at minus 12-10°C for one month and control (non-storage). There were three pre-sowing treatments as (Pre40-45) soaking seeds in 40-45°C water until reaching normal condition, (Pre70-75) soaking seeds in 70-75°C water for 5 minutes, and control (soaking seeds in normal temperature water for 5 hours). The results indicated that seeds in storage treatment cannot remain germination after storing one month, while non-storage seeds had a germination rate of 52.8%. The total germination rate (18%) was lowest in Pre70-75, increased to 20% in Pre40-45. While the highest total germination rate (52%) was found in control. It is concluded that seeds of C. impressinervis must be sown immediately after collecting from mother trees by pre-sowing treatment of soaking in normal temperature water for 5 hours and then seeds are sown in a wet sand bed.

Golden camellia, germination, healthcare, seed treatment, sowing condition

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How to Cite
THANG, N. T., LINH, M. T., MANH, T. D., DUC, D. T., HUONG, N. T. T., PHUONG, N. T. T., & DO, T. V. (2020). EFFECTS OF STORAGE AND PRE-SOWING TREATMENT ON SEED GERMINATION OF Camellia impressinervis. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(1-2), 77–83. Retrieved from http://ikprress.org/index.php/PCBMB/article/view/4927
Original Research Article


Cao J. Collected species of the genus Camellia an illustrated outline. Zhejiang Science and Technology Publishing House; 2005.

Tran DM, Nguyen TT, Hoang TS Dang VT, Phung DT, Nguyen VT, Dao TD, Mai TL, Vu TL, Nguyen HT, Nguyen TTP, Tran VD. Golden camellias: A review. Archives of Current Research International. 2019;16:1–8.

Hung TC, L Ye. Camellia impressinervis Hung T. Chang & S. Ye Liang. Acta Scientiarum Naturalium Universitatis Sunyatseni. 1979;18:72.

Hansen WM. A personal view on camellias. The Third International Academic Forum on Yellow Camellias. 2013;17–19.

Nguyen VT, Phung DT, Tran DM, Nguyen TT, Dang VT, Dao TD, Mai TL, Nguyen TTP, Ninh VK, Vu TL, Nguyen HT, Hoang TS, Trinh NB, Ho TL, Vu VT, Tran VD. Possible planting areas for golden camellia - Camellia impressinervis in Vietnam. Asian Journal of Agriculture and Horticultural Research. 2019;3:1–7.

Dao TD, Mai TL, Tran DM, Dang VT, Ly TTH, Nguyen VT, Phung DT, Nguyen TTP, Ninh VK, Dang HH, Tran CN, Tran HQ, Pham DS, Vu TL, Nguyen HT, Hoang TS, Trinh NB, Ho TL, Tran AH, Duong QT, Nguyen QH, Tran HV, Nguyen THA, Dinh HD, Vu VT, Tran VD. Cutting size and position affect rooting efficiency of Camellia impressinervis: A golden camellia. Plant Cell Biotechnology and Molecular Biology. 2019;20:179– 187.

Wan CP, Yu YY, Zhou SR, Cao SW. Antioxidant and free radical scavenging activity of Camellia nitidissima Chi. Asian Journal of Chemistry. 2011;23:2893– 2897.

Wei JB, Li X, Song H, Liang YH, Pan YZ, Ruan JX, Qin X, Chen YX, Nong CL, Su ZH. Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach. Journal of Food and Drug Analysis. 2015;23:40–48.

He D, Wang X, Zhang P, Luo X, Li X, Wang L, Li S, Xu S. Evaluation of the anxiolytic and antidepressant activities of the aqueous extract from Camellia euphlebia Merr. ex Sealy in Mice. Evidence-Based Complementary and Alternative Medicine. 2015;Article ID 6184098.

Guangxi Institute of Botany. Guangxi Flora. Guangxi Science and Technology Press: Nanning; 1991.

Wheeler L, MC Rivers. Camellia impressinervis. The IUCN Red List of Threatened Species; 2015.

Addae-Kagyah KA, Osafo DM, Olympio NS, Atubra OK. Effect of seed storage, heat pretreatment and its duration on germination and growth of nursery stock of the idolatrica palm. Elaeis guineensis var. idolatrica (chevalier). Tropical Agriculture. 1998;65:77–83.

El-Juhany LL, Aref IM, Al-Ghamdi MA. Effects of different pre-treatments on seed germination and early establishment of the seedlings of Juniperus procera trees. World Applied Science Journal. 2009;7:616–624.

Martine BM, Laurent KK, Pierre BJ, Eugene KK, Hilaire KT, Justin KY. Effect of storage and heat treatments on the germination of oil palm (Elaeis guineensis Jacq.) seed. African Journal of Agriculture Research. 2009;4:931–937.

Amoakoh OA, Nortey DDN, Sagoe F, Amoako PK, Jallah CK. Effects of pre-sowing treatments on the germination and early growth performance of Pouteria campachiana. Forest Science and Technology. 2017;3:83–86.

Alamgir M, Hossain MK. Effect of pre-sowing treatments on germination and initials seedling development of Albizia saman in the nursery. Journal of Forest Research. 2005;16:200–204.

Olatunji D, Maku OJ, Odumefun OP. The effect of pretreatments on the germination and early seedlings growth of Acacia auriculiformis Cunn. Ex. Benth. African Journal of Plant Science. 2013;7:325–330.

Hossain MA, Arefin MK, Khan BM, Rahman MA. Effects of seed treatments on germination and seedling growth attributes of horitaki (Terminalia chebula Retz.) in the nursery. Research Journal of Agriculture, Biology and Science. 2005; 1:135–141.

Bethke PC, Libourel IGL, Jones RL. Nitric oxide reduces seed dormancy in Arabidopsis. Journal of Experimental Botany. 2006;57:517–526.

De Gara L, De Pinto MC, Moliterni V, D’Egidio MG. Redox regulation and storage processes during maturation in kernels of Triticum durum. Journal of Experimental Botany. 2003;54:249–258.

Chen Q, Yang L, Ahmad P, Wan X, Hu X. Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation. Planta. 2011;233:583–92.

Chandel KPS, Chaudhury R, Radhamani J, Malik SK. Desiccation and freezing sensitivity in recalcitrant seeds of tea, cocoa and jackfruit. Annals of Botany. 1995;76: 443–450.

Roach T, Beckett RP, Minibayeva FV, Colville L, Whitaker C, Chen HY, Bailly C, Kranner I. Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds. Plant, Cell & Environment. 2010;33:59–75.

Martine BM, Laurent1 KK, Pierre BJ, Eugene KK, Hilaire KT, Justin KY. Effect of storage and heat treatments on the germination of oil palm (Elaeis guineensis Jacq.) seed. African Journal of Agriculture Research. 2009;4:931–937.

Doren ET. Vegetable ivory and other palm nuts/seeds as an art/craft medium. Principes. 1997;41:184–189.

Generalao ML. Effect of pre-sowing treatment media on the germination of Palasan (Calamus muximus Blanco) and Limuran (C. ornatus Blanco) seeds at Pagbilao, Quezon. Sylvatrop Philipp. Forest Research Journal. 1977;2:215– 218.

Airi S, Bhatt ID, Rawal RS, Dhar U. Variations in seed germination of Hippophae salicifolia with different presoaking treatments. Journal of Forest Research. 2009;20:27–30.

Azad MS, Musa ZA, Martin MA. Effect of pre-sowing treatments on seed germination of Melia azedarach. Journal of Forestry Research. 2010;21:193–196.

Missanjo E, Chioza A, Kulapani C. Effects of different pretreatments to the seed on seedling emergence and growth of Acacia polyacantha. International Journal of Forestry Research. 2014;Article ID 583069:6.

Mwase WF, Mvula T. Effect of seed size and pre-treatment methods of Bauhinia thonningii Schum. on germination and seedling growth. African Journal of Biotechnology. 2011;10:5143–5148.

Ali M, Akhter S, Kamaluddin M. Study on the bearing of hot water treatment on seed germination and seedling growth of Albizia procera Benth. Indian Forestry. 1997;123: 764–768.

Azad MS, Islam MW, Matin MA, Musa ZA. Effect of pre-sowing treatment on seed germination of Albizia lebbeck (L.) Benth. South Asian Journal of Agriculture. 2006; 1:32–34.

Mai TL, Dao TD, Tran DM, Nguyen TT, Dang VT, Nguyen TTP, Tran VD. Can Camellia japonica grow in Vietnam? Seed germination and initial growth. Plant Cell Biotechnology and Molecular Biology. 2019;20:534–540.