BIODEGRADATION OF CHLORPYRIFOS PESTICIDES BY Xanthomonas BACTERIA ISOLATED FROM AGRICULTURAL SOIL IN BAGHDAD

Main Article Content

KAIS KASSIM GHAIMA
NADHUM SHAMKHI RAHAL
BASIM SHAMKHI RAHAL
MAHIR MAHMOUD MOHAMED

Abstract

The organophosphate compound, chlorpyrifos, is widely used as an insecticide. Excessive use of these pesticides is disturbing the ecosystems and using biodegradation for their removing is a persistent need. In the present study, chlorpyrifos contaminated soil samples were collected from several agricultural fields in Baghdad city and the bacterial isolates were obtained by enrichment culture technique. Based on morphological, biochemical characteristics, 24 isolates were identified as Xanthomonas sp. The effect of different concentrations (100, 200, 300, 400, 500 and 1000 ppm) of chlorpyrifos on the total viable count of the isolates and their tolerance was carried out in this study. Four isolates (KNX5, KNX12, KNX21 and KNX23) could tolerate up to 400-1000 ppm of chlorpyrifos amended in minimal salt medium plates. The isolate KNX5 demonstrated heavy growth even at the highest concentration of 1000 ppm and used for further studies. The different culture conditions like carbon sources, the concentration of chlorpyrifos, pH and inoculum densities were used to evaluate the biodegradation potential of KNX5 strain. The good carbon source for chlorpyrifos metabolizing was glucose. The most efficient biodegradation results were obtained by using the inoculum density (108 CFU/ml) and pH (8). The strain KNX5 showed a high degradation potential of chlorpyrifos (93.1%) at the concentration of 400 mg/L after 10 days of incubation. This study indicated the importance of Xanthomonas local strains as a tool of bioremediation in the contaminated soil with chlorpyrifos.

Keywords:
Biodegradation, Chlorpyrifos, Xanthomonas.

Article Details

How to Cite
GHAIMA, K. K., RAHAL, N. S., RAHAL, B. S., & MOHAMED, M. M. (2020). BIODEGRADATION OF CHLORPYRIFOS PESTICIDES BY Xanthomonas BACTERIA ISOLATED FROM AGRICULTURAL SOIL IN BAGHDAD. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(1-2), 84–90. Retrieved from http://ikprress.org/index.php/PCBMB/article/view/4931
Section
Original Research Article

References

Aktar MW, Sengupta D, Chowdhury A. Impact of pesticides use in agriculture: their benefits and hazards. Inter. Discip. Toxicol. 2009;2(1):1–12.

Tuzmen N, Candan N, Kaya E, Demiryas N. Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defense mechanisms and lipid peroxidation in rat liver. Cell Biochem Funct. 2008;26:119–124.

Singh BK, Walker A, Morgan JAW, Wright DJ. Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl. Environ. Microbiol. 2004;70:4855–4863.

Nazia S, Raipat BS, Sinha MP. Effect of organophosphorous insecticide on soil bacteria. The Biscan. 2010;1:239-46.

Damalas CA. Understanding benefits and risks of pesticide use. Scientific Research and Essay. 2009;4(10):945-949.

Horne I, Sutherland TD, Harcourt RL, Russell RJ, Oakeshott JG. Identification of an opd (organophosphate degradation) gene in an Agrobacterium isolates. Appl. Environ. Microbiol. 2002;68:3371-76.

Yang C, Liu N, Guo X, Qiao C. Cloning of mpd gene from a Chloropyrifosdegrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett. 2006;265:18-25.

Robertson LN, Chandler KJ, Stickley BDA, Cocco RF, Ahmetagic M. Enhanced microbial degradation implicated in rapid loss of Chlorpyriphos from the controlled release formulation Sucon (R) Blue in soil. Crop Prot. 1998;17:29-33.

Rayu S, Nielsen RN, Nazaries L, Singh BK. Isolation and molecular characterization of novel chlorpyrifos and 3,5,6-trichloro-2- pyridinol-degrading bacteria from sugarcane farm soils. Front. Microbiol. 2017;8:518.

Baishya K, Sharma H. Isolation and characterization of organophosphorus pesticide degrading bacterial isolates. Arch. Appl. Sci. Res. 2014;6(5):144-149.

Akbar S, Sultan S. Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement. Brazilian Journal of Microbiology. 2016;47(3):563-570.

Supreeth M, Chandrashekar MA, Sachin N, Raju NS. Effect of chlorpyrifos on soil microbial diversity and its biotransformation by Streptomyces sp. HP-11. Biotech. 2016;6(2):147.

Holt GH, Krieg NR, Sneath PNA, Staley JT, Williams ST. Gram positive cocci. In: Bergey’s Manual of Determinative Bacteriology. 9th edition. William and Wilkins Baltimore Maryland, U.S.A. 1994; 527-533.

Greenberg AE, Clesceri LS, Eaton AD. Standard methods for the examination of water and waste water. 18th ed. APHA, Washington; 1992.

Schaad NW, Jones JB, Chun W. Laboratory guide for identification of plant pathogenic bacteria, Third Edition. American Phytopathological Society Press, St Paul, USA; 2001.

Hauben L, Vauterin L, Swings J, Moore ER. Comparison of 16S ribosomal DNA sequences of all Xanthomonas species. Int. J. Syst. Bacteriol. 1997;47(2):328-35.

Zhu J, Zhao Y, Qiu J. Isolation and application of a chlorpyriphos-degrading Bacillus licheniformis ZHU-1. Afri. J. Microbiol. Res. 2010;4(22):2410-2413.

Singh BK, Walker A. Microbial degradation of organophosphorus compounds. FEMS Microbiol. Rev. 2006;30:428–471.

Khalid S, Hashmi I, Khan SJ. Bacterial assisted degradation of chlorpyrifos: The key role of environmental conditions, trace metals and organic solvents. Journal of Environmental Management. 2016;168:1–9.

Chawla N, Suneja S, Kukreja K. Isolation and characterization of chlorpyriphos degrading bacteria. Indian J. Agric. Res. 2013;47:381–391.

Friyah SH, Rasheed MN. Molecular study of efflux MexX gene in Pseudomonas aeruginosa isolated from Iraqi patients. Iraqi Journal of Biotechnology. 2018;17(3):39-41.

Yu CP, Yu YW. Evaluation of biodegrability by the reduction of tetrazoluim violet in biolog microplates. Biotechnol. Lett. 2000;22:909-919.

Sarkar S, Seenisavan S, Asir RPS. Biodegradation of propargite by Pseudomonas putida, isolated from tea rhizosphere. J. Hazard Mater. 2010;174(1-3):295-8.

Fang H, Xiang YJ, Hao XQ, Chu XD, Pan JQ, Yu JQ, Yu YL. Fungal degradation of chlorpyrifos by Verticillium sp. DSP in pure cultures and its use in bioremediation of contaminated soil and pakchoi. Int. Biodeter. Biodegrad. 2008;61:294-303.

Bhattacharya J, Islam M, Cheong YW. Microbial growth and action: Implications for passive bioremediation of acid mine drainage. J. Mine Water. Environ. 2006;25:233-240.

Atul B, Neelma V. Proficient biodegradation studies of Clorpyrifos and Its metabolite 3, 5, 6- Trichloro-2-pyridinol by Bacillus subtilis NJ11 Strain. Research Journal of Microbiology. 2018;13:53-64.

Saunders M, Magnanti BL, Correia S, Alamo-Hernandes U. Chlorpyrifos and neurodevelopmental effects: A literature review and expert elicitation on research and policy. Environ Health. 2012;11(Suppl 1):S5.