In vitro Micropropagation of Vitis vinifera L. var. Cabernet Franc and Callus Production

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Published: 2023-04-15

Page: 30-38


Yanina S. García

Facultad de Química Bioquímica y Farmacia, Laboratorio de Fisiología Vegetal, Área de Ecología, PROICO 02-3318, Universidad Nacional de San Luis, Ejército de los Andes 950 (5700), San Luis, Argentina.

Evelyn A. González

Facultad de Química Bioquímica y Farmacia, Laboratorio de Fisiología Vegetal, Área de Ecología, PROICO 02-3318, Universidad Nacional de San Luis, Ejército de los Andes 950 (5700), San Luis, Argentina.

Olga M. Ruiz

Facultad de Ingeniería y Ciencias Agropecuarias, Universidad Nacional de San Luis, Autopista 55 Extremo Norte (5730), Villa Mercedes, San Luis, Argentina.

M. Cecilia Pacheco Insausti

Facultad de Química Bioquímica y Farmacia, Laboratorio de Fisiología Vegetal, Área de Ecología, PROICO 02-3318, Universidad Nacional de San Luis, Ejército de los Andes 950 (5700), San Luis, Argentina.

Mariano H. Gutiérrez

Facultad de Ingeniería y Ciencias Agropecuarias, Universidad Nacional de San Luis, Autopista 55 Extremo Norte (5730), Villa Mercedes, San Luis, Argentina.

Eliana S. Fernandez

Facultad de Ingeniería y Ciencias Agropecuarias, Universidad Nacional de San Luis, Autopista 55 Extremo Norte (5730), Villa Mercedes, San Luis, Argentina.

Hilda E. Pedranzani

Facultad de Química Bioquímica y Farmacia, Laboratorio de Fisiología Vegetal, Área de Ecología, PROICO 02-3318, Universidad Nacional de San Luis, Ejército de los Andes 950 (5700), San Luis, Argentina.

*Author to whom correspondence should be addressed.


Abstract

The objective of this study was to produce in vitro-cultured mother plants of Cabernet Franc. Uninodal cuttings were taken from the plants, disinfected and sown in 50% Murashige and Skoog (MS) medium with the addition of 0.01% indoleacetic acid (IAA). Explant establishment, rooting, and leaf number were recorded and statistically analyzed after eight weeks of growth. The ex-vitro behavior of the plants was analyzed after four weeks of acclimatization under appropriate conditions of humidity and temperature in a 1:1 mixture of perlite: sterile fertile soil. The result showed that it is an optimal variety for in vitro culture, with mean values of 88% of establishment and 79% of rooting, production of 5 leaves per cutting and 100% of ex vitro acclimatization. Genetic material with good yield, health and acclimatization was obtained from the in vitro production of mother plants of Cabernet Franc. The Cabernet Franc explants formed callus after 15 days of exposure to 2,4-D. This indicates that it is an optimal material for genetic improvement.

Keywords: Acclimatization, callus, In vitro culture, micropropagation, rooting


How to Cite

García, Y. S., González, E. A., Ruiz, O. M., Insausti, M. C. P., Gutiérrez, M. H., Fernandez, E. S., & Pedranzani, H. E. (2023). In vitro Micropropagation of Vitis vinifera L. var. Cabernet Franc and Callus Production. Asian Journal of Agriculture and Allied Sciences, 6(1), 30–38. Retrieved from https://ikprress.org/index.php/AJAAS/article/view/8204

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