Asian Journal of Microbiology and Biotechnology

The present research trial was conducted at the School of Agriculture, Loyola Academy Degree & P.G College, during the Kharif season 2024, to determine the effective yield of oyster mushroom production using locally available substrates, with four treatments (paddy straw, coconut coir, cardboard paper, and sawdust), each replicated 3 times in a CRD experimental design. The project data analysis revealed that T₁ (paddy straw) was more efficient than the other treatments, namely T₂ (coconut coir), T₃ (sawdust), and T₄ (cardboard). Analysis of variance showed a significant difference @ 1 % and 5 % level of significance for all the parameters, but high significance was observed for paddy straw treatment i.e., Treatment 1 (control) paddy straw is 45 for the number of Pinheads, 0.363 for stalk diameter, pileus length 2.167 cm, Pileus thickness 0.65 cm, 1.9 cm for less stalk length, highest yield is 21 grams was observed during the experiment respectively. In the analysis part of the mushroom composition from various substrates, mushrooms from the paddy straw have given the highest amount of proteins and amino acids. The mushrooms absorb the soluble sugar compared to the lignocellulosic substances, and these sugars are mainly used for growth and other metabolic activities.