In-vitro ANTIFUNGAL STUDY OF CRUDE EXTRACT OF Barringtonia asiatica (L.) Kurtz AGAINST FUNGAL PATHOGENIC STRAINS
ISAAC JOHN UMARU *
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia and Department of Biochemistry, Federal University Wukari, Taraba State, Nigeria.
FASIHUDDIN BADRUDDIN AHMED
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia.
KERENHAPPUCH ISAAC UMARU
Department of Biochemistry, University of Maiduguri, Borno State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Barringtonia asiatica (L.) Kurtz belong to a Family of Lecythidaceae is a species native to mangrove habitats in the tropical. The aim of this study was to evaluate the In-vitro Antifungal Study of Crude extract of Barringtonia asiatica (L.) Kurtz against Fungal Pathogenic Strains.
Materials and Methods: Extractions was carried out through conventional method, using cold-soaking method in non-polar, medium polar and polar solvents in the order of increasing polarity (hexane, dichloromethane, chloroform, ethyl acetate and methanol). Antifungal activity was evaluated using disc diffusion assay, using Potato dextrose agar medium (PDA), Diflucan as control and Candida tropicalis, Fusarium oxysporum, Aspergillus niger and Aspergillus flavin as pathogen were utilized.
Results: Significant inhibition of hexane extract was observed on Aspergillus niger at 500 µg/mL with inhibition zone of 14.77±0.05 mm. Dichloromethane crude leaf extract exhibited higher growth inhibition against Fusarium oxysporium with inhibition zone of 15.93 ± 0.05 mm.
Conclusion: The focus of this study is to provide the prospects of Barringtonia asiatica different solvent from non-polar and polar crude extract as a good agent for combating antifungal menace and diseases resistance from modern medicine.
Keywords: Polar, non-polar, in-vivo, antifungal, Aspergillus niger, dextrose ager, Candida tropicalis.