STUDY OF ANTIMICROBIAL PEPTIDES EXTRACTED FROM CYANOBACTERIUM, Spirulina sp.

Full Article - PDF

Published: 2021-08-03

Page: 10-17


A. H. DEEPIKA *

B. S. Abdur Rahman Crescent Institute of Science and Technology, India.

*Author to whom correspondence should be addressed.


Abstract

Marine microalgae are specifically noteworthy right now they have created resilience and safeguard procedures to oppose the introduction to pathogenic microbes, infections, and growths in the aquatic environment. Microalgae are quite compelling because of their gigantic biodiversity and their generally simple development needs. Spirulina is the most explored microalgae due to its commercial importance as as a source of protein, vitamins, amino acids, and fatty acids. It is a blue-green multicellular microalgae attributable to chlorophyll (green) and phycocyanin (blue) shades in its concoction piece. The protein-rich Spirulina species is inoculated in Zarrouk’s medium. In this study, the biomass of Spirulina sp is increased for extraction of peptide fractions. Acid extraction is the method employed for the extraction of antimicrobial peptides from the Spirulina sp. Moreover, sonication is carried out to disrupt the cell membrane. This process is used to enhance the process of extraction. Sonication uses probe to apply sound energy to a liquid containing particles. Furthermore, the extracted peptide can be bound to solid-phase extraction and active fractions can be obtained and purified by HPLC. The peptide sequence can be identified by mass spectroscopic techniques and can be further subjected to antimicrobial activity in future studies.

Keywords: Spirulina sp, antimicrobial peptides, acid extraction, solid-phase extraction


How to Cite

DEEPIKA, A. H. (2021). STUDY OF ANTIMICROBIAL PEPTIDES EXTRACTED FROM CYANOBACTERIUM, Spirulina sp. Asian Journal of Microbiology and Biotechnology, 6(1), 10–17. Retrieved from https://ikprress.org/index.php/AJMAB/article/view/6784

Downloads

Download data is not yet available.

References

Adiba Benahmed Djilali, et al. Preliminary characterization of food tablets from date (Phoenix dactylifera L.) and Spirulina (Spirulina sp.) powders. Powder Technol. 2011;208(3):725-730.

Guzmán Fanny, et al. Identification of antimicrobial peptides from the microalgae Tetraselmis suecica (Kylin) Butcher and bactericidal activity improvement. Mar. Drugs. 2019;17(8):453.

Zheng Jiahui, Jingyue Wang, Huanglei Pan, Hongli Wu, Difeng Ren, Jun Lu, et al. Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats. Mol. Med. Rep. 2017;16(6):8485-8492.

Finamore Alberto, Maura Palmery, Sarra Bensehaila, aIlaria Peluso, et al. Antioxidant, immunomodulating, and microbial-modulating activities of the sustainable and ecofriendly Spirulina. Oxi Med Cell Longev; 2017.

Sun Yijun, et al. Isolation and characterization of an antibacterial peptide from protein hydrolysates of Spirulina platensis. Eur. Food Res. Technol. 2016;242(5):685-692.

Akao Yuusuke, Takashi Ebihara, Hisayo Masuda, Yoshiko Saeki, Takashi Akazawa, Kaoru Hazeki, Osamu Hazeki, Misako Matsumoto, Tsukasa Seya, et al. Enhancement of antitumor natural killer cell activation by orally administered Spirulina extract in mice. Cancer Sci. 2009;8:1494-1501.

Powers Jon-Paul S, Robert EW Hancock, et al. The relationship between peptide structure and antibacterial activity. Peptides. 2003;24(11):1681-1691.

Welker Martin, aHans Von Döhren, et al. Cyanobacterial peptides—nature's own combinatorial biosynthesis. FEMS Microbiol. 2006;30(4):530-563.

Lu Congming, Avigad Vonshak, et al. Effects of salinity stress on photosystem II function in cyanobacterial Spirulina platensis cells. Physiol. Plant. 2002;114(3):405-413.

Mallikarjun Gouda KG, Kavitha MD, Sarada R, et al. Antihyperglycemic, Antioxidant and antimicrobial activities of the butanol extract from spirulina platensis. J. Food Biochem. 2015;39(5):594-602.

Verdasco-Martín Carlos M, Cristina Otero, et al. Advantageous preparation of digested proteic extracts from Spirulina platensis biomass. Catalysts. 2019;9(2):145.

Pan H, She X, Wu H, Ma J, Ren D, Lu J, et al. Long-term regulation of the local renin–angiotensin system in the myocardium of spontaneously hypertensive rats by feeding bioactive peptides derived from Spirulina platensis. J. Agric. Food Chem. 2015;63(35):7765-7774.

Czerwonka Arkadiusz, Katarzyna Kaławaj, Adrianna Sławińska-Brych, Marta K Lemieszek, Magdalena Bartnik, Krzysztof K Wojtanowski, Barbara Zdzisińska, Wojciech Rzeski, et al. Anticancer effect of the water extract of a commercial Spirulina (Arthrospira platensis) product on the human lung cancer A549 cell line. Biomed Pharmacother. 2018;106:292-302.

Vo Thanh-Sang, BoMi Ryu, Se-Kwon Kim. Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal. J. Funct. 2013;5(3):1336-1346.

Heo Seong-Yeong, et al. A heptameric peptide purified from Spirulina sp. gastrointestinal hydrolysate inhibits angiotensin I-converting enzyme-and angiotensin II-induced vascular dysfunction in human endothelial cells. Int. J. Mol. Med. 2017;39(5):1072-1082.

Carrizzo Albino, et al. Novel potent decameric peptide of Spirulina platensis reduces blood pressure levels through a PI3K/AKT/eNOS-dependent mechanism. Hypertension. 2019;73(2):449-457.

Zhang Bochao, Xuewu Zhang, et al. Separation and nanoencapsulation of antitumor polypeptide from Spirulina platensis. Biotechnol. 2013;29(5):1230-1238.

Wang Zhujun, Xuewu Zhang, et al. Isolation and identification of anti‐proliferative peptides from Spirulina platensis using three‐step hydrolysis. J. Sci. Food Agric. 2017;97(3):918-922.

Kim Nam-Ho, et al. Purification of an iron-chelating peptide from Spirulina protein hydrolysates. J KOREAN SOC APL BI 57. 2014;1:91-95.

Zeng Qiaohui, et al. Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis. J. Appl. Soc. Psychol. 2018;30(3):1655-1665.

Sannasimuthu Anbazahan, et al. Design and characterization of a novel Arthrospira platensis glutathione oxido-reductase-derived antioxidant peptide GM15 and its potent anti-cancer activity via caspase-9 mediated apoptosis in oral cancer cells. Free Radic. 2019;135:198-209.

Fan Xiaodan, Yujiao Cui, Ruilin Zhang, Xuewu Zhang, et al. Purification and identification of anti-obesity peptides derived from Spirulina platensis. J.Funct. Foods. 2018;47:350-360.

Mitta Guillaume, et al. Myticin, a novel cysteine‐rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. Eur J Biochem. 1999;265(1):71-78.