STUDY OF ANTIMICROBIAL PEPTIDES EXTRACTED FROM CYANOBACTERIUM, Spirulina sp.
A. H. DEEPIKA *
B. S. Abdur Rahman Crescent Institute of Science and Technology, India.
*Author to whom correspondence should be addressed.
Abstract
Marine microalgae are specifically noteworthy right now they have created resilience and safeguard procedures to oppose the introduction to pathogenic microbes, infections, and growths in the aquatic environment. Microalgae are quite compelling because of their gigantic biodiversity and their generally simple development needs. Spirulina is the most explored microalgae due to its commercial importance as as a source of protein, vitamins, amino acids, and fatty acids. It is a blue-green multicellular microalgae attributable to chlorophyll (green) and phycocyanin (blue) shades in its concoction piece. The protein-rich Spirulina species is inoculated in Zarrouk’s medium. In this study, the biomass of Spirulina sp is increased for extraction of peptide fractions. Acid extraction is the method employed for the extraction of antimicrobial peptides from the Spirulina sp. Moreover, sonication is carried out to disrupt the cell membrane. This process is used to enhance the process of extraction. Sonication uses probe to apply sound energy to a liquid containing particles. Furthermore, the extracted peptide can be bound to solid-phase extraction and active fractions can be obtained and purified by HPLC. The peptide sequence can be identified by mass spectroscopic techniques and can be further subjected to antimicrobial activity in future studies.
Keywords: Spirulina sp, antimicrobial peptides, acid extraction, solid-phase extraction