Asian Journal of Microbiology and Biotechnology

SCREENING AND IDENTIFICATION OF GENETIC PURITY OF OKRA HYBRID [RANI] AND ITS PARENTAL LINES BASED ON MOLECULAR MARKERS

Asian Journal of Microbiology and Biotechnology, Volume 7, Issue 1, Page 37-45
DOI: 10.56557/ajmab/2022/v7i17884

Abstract


The present investigation was carried out to identify the genetic purity of okra hybrid and its parental line based on molecular markers. For the evaluation of genetic purity and varietal identification from collected Okra samples, molecular markers and polymerase chain reaction, as well as DNA fingerprinting approaches, were advocated. These samples were accumulated from field and nursery Adithya seeds Pvt. Ltd, Raipur (C.G) within the duration of study and research from January to July 2018. Genetic purity test over the conventional Grow-Out-Test (GOT) was achieved by using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) primers for identifying and emphasizing of hybrid. Using 3 lines of Okra cultivars, we analysed the products of PCR with RAPD and ISSR primers and discussed the design for constructing their Genetic Purity. Towards this study 64 random primers were screened between okra hybrid (Rani) and its parental lines [male line (206) and female line (189)]. Out of 64 primers only one primer (ISSR-B17898) showed polymorphic banding pattern between hybrid and its parental lines which helped in the identification and confirmation of okra hybrid. Hence, concluded that Genetic purity test is necessary for seed certification of different categories in seed multiplication chain both from agronomic as well as breeding point of view and proved to be explicit technology for researchers.

Keywords:
  • Okra
  • genetic purity
  • hybrid
  • molecular markers
  • PCR

How to Cite

LAKRA, V., DAS, P., & DAS, P. (2022). SCREENING AND IDENTIFICATION OF GENETIC PURITY OF OKRA HYBRID [RANI] AND ITS PARENTAL LINES BASED ON MOLECULAR MARKERS. Asian Journal of Microbiology and Biotechnology, 7(1), 37–45. https://doi.org/10.56557/ajmab/2022/v7i17884

References

Bates DM. Notes on the cultivated Malvaceae 2. Abelmoschus. Baileya. 1968;16:99-112.

Düzyaman E. Okra: Botany and Horticulture. In: Horticultural Review, Janick, J., ed.; John Wiley and Sons. 1997; 41-72.

De Candolle A. Origin of cultivated plants. NY:Hather, New York;1886.

Grubben GJH. Tropical vegetables and their genetic resources, Tindall H.D., Willams J.T., Eds.; IBPGR. Rome; 1977.

Kumar S, Dagnoko S, Haougui A, Ratnadass A, Pasternak D, Kouame C. Okra (Abelmoschus spp.) in West and Central Africa: potential and progress on its improvement. Afr. J. Agric. Res. 2010;5(25):3590-3598. [Google Scholar]

Indian Horticulture Database 2009. Bijay Kumar., Eds.; NHB:National Horticulture Board, Gurgaon, India. 2010; 154-161.

Schafleitner R, Kumar S, Lin CY, Hegde SG, Ebert A. The okra (Abelmoschus esculentus) transcriptome as a source for gene sequence information and molecular markers for diversity analysis. Gene. 2013;517(1):27-36. [Google Scholar]

Inamullah, Ahmed H, Muhammad F, Sirajuddin, Hassan G, Gul R. Evaluation of heterotic and heterobeltiotic potential of wheat genotype for improved yield. Pak. J. Bot. 2006;38(4):1159-1168. [Google Scholar]

Reddy MT, Haribabu K, Ganesh M, Reddy KC, Begum H, Subbararama Krishna Reddy R, Dilip Babu J. Genetic analysis for yield and its components in okra (Abelmoschus esculentus (L.) Moench). Songklanakarin J. Sci Technol. 2012;34(2):133-141.

[Google Scholar]

Yen CC. Identification of the genuineness and purity of hybrid rice (Oryza sativa sp.indica) and its parental lines by electrophoresis analysis of esterase. Seed Sci. Technol. 1987;15:645-649. [Google Scholar]

Yashitola J, Thirumurugan T, Sundaram RM, Naseerullah MK, Ramesha MS, Sarma NP, Sonti RV. Assessment of purity of rice hybrids using microsatellite and STS markers. Crop Sci. 2002;42:1369-1373.

[Google Scholar]

Dogney ML. Significance of grow out test (GOT) in hybrid seed production of cotton. Res. Crops. 2002;3(1):193-194.

[Google Scholar]

Dogney ML. Significance of grow out test (GOT) in certified seed production of wheat. Res. Crops. 2005;6(2):357-358.

[Google Scholar]

Meena RK, Verma AK, Thakur S. Molecular Identity Using Inter-simple Sequence Repeat & Random Amplified Polymorphic DNA Markers in Soybean (Glycine max) Cultivars. Curr. Trends Biotechnol. Pharm. 2015; 9(1):16-22. [Google Scholar]

Dhurwe S, Ghatage T, Mishra R, Das P, Verma AK. Fingerprinting of Bottle Gourd (Lagenaria siceraria) Hybrids and their Parental Lines Using Microsatellite Markers and their Utilization in Genetic Purity Assessment, Int. J.Curr. Biotechnol. 2017;5(5):1-5

[Google Scholar]

Sawadogo M, Ouedraogo JT, Balma D, Ouedraogo M, Gowda BS, Botanga C, Timko MP. The use of cross species SSR primers to study genetic diversity of okra from Burkina Faso. Afr. J. Biotechnol. 2009;8(11):2476-2482. [Google Scholar]

Yuan CY, Zhang C, Wang P, Hu S, Chang HP, Xiao WJ, Guo XH. Genetic diversity analysis of okra (Abelmoschus esculentus L.) by intersimple sequence repeats (ISSR) markers. Genet. Mol. Res. 2014;13(2):3165-3175. [Google Scholar]

Esselman EJ, Jianqiang L, Crawford DJ, Windus JL, Wolfe AD. Clonal diversity in the rare Calamagrostis porter ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Mol. Ecol. Resour. 1999;8(3):443-451. [Google Scholar]

Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Pytochemical Bulletin. 1987;19:11-15. [Google Scholar]

Sahu S, Das P, Dwivedi K. An efficient method for the isolation of purest form of DNA from the okra plant. Indo Am. J. Pharm. Sci. 2018; 5(5):3830-3832.

[Google Scholar]

Hamon S, Van Sloten DH. Characterization and Evaluation of Okra. In: The Use of Plant Genetic Resources, Brown, A.H.D., ed.; Cambridge University Press, Cambridge. 1989; 173-174.

Sundaram RM, Naveenkumar B, Biradar SK, Balachandran SM, Mishra B, Ilyas Ahmed M, Viraktamath BC, Ramesha MS, Sarma NP. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment. Euphytica. 2008;163(2):215-224. [Google Scholar]

Lamont, Jr. WJ. Okra - A versatile vegetable crop. Hort. Technol. 1999; 9(2):179-184.
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