Histochemical Staining and SDS-PAGE Analysis of Blackgram Genotypes Subjected to Temperature Induction Response Technique
N. Pavithra
*
Department of Crop Physiology, Agricultural College, Bapatla, Acharya N.G Ranga Agricultural University, Bapatla-522101, Andhra Pradesh, India.
K. Jayalalitha
ANGRAU, RARS, Lam, Guntur-522034, India.
T. Sujatha
ANGRAU, DAATTC, Eluru-534 005, India.
N. Harisatyanarayana
ANGRAU, RARS, Lam, Guntur-522034, India.
N. Jyothi Lakshmi
CRIDA, Hyderabad-500059, India.
V. Roja
ANGRAU, RARS, Lam, Guntur-522034, India
*Author to whom correspondence should be addressed.
Abstract
The study was conducted at Department of Crop Physiology, Agricultural College, Bapatla in Acharya N.G Ranga Agricultural University. The results of the histo-chemical staining revealed that the genotypes TBG-129, PU-1804 and TBG-104 showed lesser free radical accumulation on the surface of the leaves indicating tolerance to high temperatures. The protein profile as evidenced by SDS page analysis indicated distinct expression of band between 23-30 KDa protein in tolerant genotypes such as TBG-129, PU-1804, LBG-995, PU-31, TBG-104, TBG-141, LBG-1004, LBG-1015 and LBG-996. Moreover, the band between 23-30 KDa was denser in TBG-129 and PU-1804. This expression of protein might be an indicative feature of thermotolerance.
Keywords: Blackgram, histochemical staining, SDS-PAGE, thermotolerance, temperature induction response