Molecular Insights into Bile Salt Hydrolase-Positive Lactobacilli: A PCR-Based Identification Approach
B. M. Manjunatha *
Department of Dairy Engineering, College of Dairy Technology, Sri Venkateswara Veterinary University, Tirupati, 517502, India.
N. Supraja
Department of Biotechnology, Thiruvalluvar University, Vellore, 632001, India.
B. Vijaykumar
Department of Biotechnology, Sri Venkateswara University, Tirupati, 517502, India.
Venkateswarlu Yedoti
Green Tech Biosciences India Pvt Limited, Chitvel, Annamayya, District-516128, India.
*Author to whom correspondence should be addressed.
Abstract
Bile salt hydrolase (BSH)-positive Lactobacilli play a crucial role in gut health and have been associated with various physiological benefits. This study presents a comprehensive molecular investigation into the identification of BSH-positive Lactobacilli using a polymerase chain reaction (PCR)-based approach. The methodology involves the targeted amplification of specific genetic markers associated with BSH activity, enabling the precise discrimination of these beneficial bacteria within complex microbial communities. The study encompasses the design and validation of PCR primers specific to BSH-encoding genes, ensuring the accurate detection of BSH-positive Lactobacilli strains. Utilizing this molecular tool, we conducted extensive screenings across diverse environmental samples, revealing the prevalence and diversity of BSH positive strains in various ecosystems.
Keywords: PCR, bile salt, lactobacilli, neutraceuticals