Assessment of Mutagenic Effects of Ethyl Methane Sulphonate (EMS) on Some Biological and Cytological Characteristics of Coriandrum sativum L. var. CO-2
Bilques Aashiq
School of Agricultural Science, Glocal University, Mirzapur Pole-Saharanpur-247121, India.
Rayees Afzal Mir *
School of Agricultural Science, Glocal University, Mirzapur Pole-Saharanpur-247121, India.
Mohd Gulfishan
School of Agricultural Science, Glocal University, Mirzapur Pole-Saharanpur-247121, India.
*Author to whom correspondence should be addressed.
Abstract
The current study was conducted using Coriandrum sativum L. (Coriander) Var. CO-2 seeds that were chosen. The seeds, which were identical, healthy, and dry, were exposed to varying amounts of EMS (0.1, 0.2, 0.3, and 0.4%). Study was done on germination characteristics such as seed germination proportion, seedling size, seedling damage and meiotic behavior of the chromosomes. The control group's seed germination percentage was 91%. The percentage of seeds that germinated when exposed to EMS treatments showed a gradual decline from smaller doses to higher ones. Following EMS therapy, there was a dose-dependent decrease in seedling height. The spectrum of seedling height in seeds treated with EMS was 11.81 cm to 10.30 cm, whereas the degree of seedling harm was 1.63% to 5.83%. Different chromosomal aberrations have also been recorded following the administrations various EMS treatments. A dose dependent increase in the chromosomal anomalies was recorded which suggest that meiotic behavior of chromosome was significantly affected by the EMS treatment. It can be concluded that changes, due to EMS treatment, in M1 biological parameters including meiotic behavior can be exploited to create or enhance the genetic diversity which subsequently be used in future crop improvement program through induced mutagenesis by using EMS or other mutagens.
Keywords: Coriandrum sativum L, seedling injury, EMS, meiosis, mutation breeding