Asian Journal of Current Research

Innovations in utilizing the phytonutrient content of natural ingredients, one which is P. indica leaf in whiteleg shrimp farming, has various functional benefits especially as a natural medicine for shrimp health. This study aimed to investigate the antibacterial and antioxidant potential of P. indica leaf extract and to evaluate its toxicity level toward whiteleg shrimp. Antibacterial activity was assessed using the well diffusion method, while antioxidant capacity was measured through the DPPH assay. Toxicity was determined based on the 96-hour LC₅₀ test. Phytochemical screening revealed that the P. indica leaf extract contains bioactive compounds, including alkaloids, saponins, polyphenols (flavonoids, tannins, and phenols), triterpenoids, and glycosides, which contribute to its functional properties. P. indica leaf extract exhibited an LC₅₀ value of 432.51 ppm which has toxic potential for whiteleg shrimp, provides antibacterial activity from a concentration of 1% with a weak category of 1.01 ± 0.43 mm and becomes stronger as the concentration increases with the highest result of the V. parahaemolyticus bacterial inhibition zone of 13.72 ± 1.09 mm at a concentration of 100% and was classified as a strong antioxidant agent (average EC₅₀ value of 82,630±2.76 ppm).