GENETIC DIVERSITY ANALYSIS AMONG SOME BARLEY GENOTYPES USING MULTIVARIABLE AND MOLECULAR MARKERS UNDER DIFFERENT LEVELS OF SOIL SALINITY STRESS
Asian Journal of Plant and Soil Sciences, Volume 7, Issue 1,
Page 219-236
Abstract
In Egypt, around 60% of cultivated lands of Northern Delta region are considered soil salinity areas. A two-year three field experiments were carried out to evaluate six Egyptian Barley genotypes under different soil salinity levels (4 , 8 and 12 dSm-1) during two consecutive seasons 2019/2020 and 2020/2021 using agro-morphological, germination percentage, seedling vigor, grain quality, stress indices, multivariable analysis and inter simple sequence repeats (ISSRs) marker. Increasing salinity level from (4 to 12 dSm-1) diminished the performance of all measured traits; however increasing protein content reached to 15.39% and heading data was quickens by 5.44% with strong and negative relationship with grain yield. Six salt stress indices were calculated based on grain yield; results showed that mean productivity (MP), harmonic Mean (HM) and stress tolerance index (STI) were more effective indices for detecting the high yielding genotypes. Ten ISSR primers were used to assess the genetic diversity among all genotypes; all primers showed high average percentage of polymorphic loci was 81.6%. The ISSR (UBC 835) primer generate the highest band number, polymorphism, polymorphism information content (PIC), effective multiplex ratio (EMR), diversity index (DI),), resolving power (RP), and marker index MI values were (8,100%, 0.50, 0.43, 5.80 3.20 and 0.88) respectively. Furthermore, ISSR (UBC 835) primer had amplified specific allele with molecular size 800 bp found in the tolerance cultivars (Line 4 and Giza 137) indicating that this primer is highly informative as a positive marker for salt tolerance. The heatmap cluster constructed using Euclidean distance and average linkage based on 12 phenotypic traits, seven salt tolerance indices and 10 ISSR primers showing that the six Barley genotypes were clustered into two main clusters, each cluster include the most closed genotypes together based on their response to salinity stress which could be used future in salinity breeding programs for high yield production under salinity soil areas in Egypt.
Keywords:
- Barley
- viability parameters
- agro-morphological traits
- germination percentage
- seedling vigor
- grain quality
- stress indices
- GT-Biplot
- correlation
- ISSR markers
- PCA-Biplot cluster heatmap
How to Cite
MARIEY, S. A., GHAREEB, Z. E., SHAHEIN, A. M. E. A., MELEHA, A. M. I., GOMAA, H. A., RADY, A. M. S., & KHATAB, I. A. (2022). GENETIC DIVERSITY ANALYSIS AMONG SOME BARLEY GENOTYPES USING MULTIVARIABLE AND MOLECULAR MARKERS UNDER DIFFERENT LEVELS OF SOIL SALINITY STRESS. Asian Journal of Plant and Soil Sciences, 7(1), 219–236. Retrieved from https://ikprress.org/index.php/AJOPSS/article/view/7600
References
Sarraf M, Vishwakarma K, Kumar V, Arif N, Das S, Johnson R, Janeeshma E, Puthur JT, Aliniaeifard S, Chauhan DK. Metal/ metalloid-based nanomaterials for plant abiotic stress tolerance: An overview of the mechanisms. Plants. 2022;11:316.
Waqas Manzoor Bhutta. Identification of salinity resistance wheat (Triticum aestivum L.) genotypes by SSR markers. Biomed J Sci & Tech Res. 2021;35:202.
FAO. Land and Plant Nutrition Management Services; 2008. Available:http://www.fao.org/agb/agl/agll/spush/
Egyptian Environmental Affairs Agency (EEAA). Egypt National 3rd communication Under UNFCCC. Cairo: Egyptian Environmental Affairs Agency (EEAA) (2016).
FAOSTAT, D. Food and Agriculture Organization of the United Nations. Statistical Database. FAOSTAT. 2021;XVII. Available:http://www.fao.org/faostat/en/#home (accessed on 12 January 2021).
Mariey Samah A, Amany M. Mohamed and Heba G. Ali. Effect of salinity stress on physiological and biochemical traits of Barley cultivars. International Journal of Environment. 2018a;7: 65-77.
Hammami Z, Qureshi A, Sahli A, Gauffreteau A, Chamekh Z, Ben Azaiez Fatma E, Ayadi S, Trifa Y. Modeling the effects of irrigation water salinity on growth, yield and water productivity of barley in three contrasted environments. Agronomy. 2020;10:1459.
Awad A, Odat N, Abu-Romman S, Hasan M, Al-Tawaha AR. Effect of salinity on germination and root growth of Jordanian Barley. Journal of Ecological Engineering. 2021;22:41.
Mariey, Samah A, Khedr Rania A, Zayed, B. A., and Elakhdar A. Genetic Variability among Egyptian Barley varieties for agro- morphological traits under saline soil condition .Egypt. J. Plant Breed. 2017;21:577.
Moustafa, Ehab SA. Assessment of genetic variations and interrelationships among agronomic traits in advanced breeding Barley lines under salinity condition. Egyptian J. Desert Res. 2021;71:1-22.
Mansour E, Moustafa S, Mohamed IE, Abdul-Hamid, Salwa MAI. Ash-shormillesy, Abdel-Rahman MA. Merwad, Hany AW, Ernesto I. Field responses of Barley genotypes across a salinity gradient in an arid mediterranean environment. Agricultural Water Management. 2021;258:107-206.
Jamshidi A, Javanmard HR. Evaluation of Barley (Hordeum vulgare L.) genotypes for salinity tolerance under field conditions using the stress indices. Ain Shams Eng J. 2018;9: 2093:2099.
Mariey Samah A, Ahmed KR, Agwa AME, Farid Mona. A, Serag AM. Biochemical and molecular genetic markers associated with salt stress tolerance in Egyptian Barley cultivars. Egypt. J. Plant Breed. 2019;23:183.
Allel D, BenAma A, Badri r, M, Abdelly C. Evaluation of salinity tolerance indices in North African Barley accessions at reproductive stage. Czech J. Genet. Plant Breed. 2019;55:61.
Moustafa ESA, Ali MMA, Kamara MM, Awad MF, Hassanin AA, Mansour E. Field screening of wheat advanced lines for salinity tolerance. Agronomy. 2021;11:281.
Oubaidou R, Hentour S, Houasli C, Aboutayeb, R, El Goumi Y, El Maaiden E, Fakiri M. Evaluating salt tolerance in doubled haploid Barley lines using a multivariable screening approach. Biocatalysis and Agricultural Biotechnology. 2021;35:102060.
Mariey Samah A, Mohamed Eman N, Ghareeb Zeinab E, Abo Zaher Engy S. Genetic diversity of Egyptian Barley using agro–physiological Traits, grain quality and molecular markers. Current Science International. 2021;10:58.
Godwin ID, Aitken EA, Smith LW. Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis. 1997;18:1524.
Reddy MP, Sarla N, Siddiq E. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica. 2002; 128:9–17.
Mareiy A. Samah, Mona A. Farid and karima A. R) Morphological and Molecular Characterization of Some Egyptian Barley Cultivars under Calcareous Soil conditions. Middle East Journal of Agriculture Research. 2018b , 07: 408- 420
Velicevici G, Madoşă E, Ciulca S, Camen D, Ciulca A, Petolescu C, Malaescu M, , Nistor E, Beinsan C. Analysis of genetic diversity in Barley cultivars using ISSR markers. Journal of Horticulture, Forestry and Biotechnology. 2018;22:19.
Khatab IA, El-Mouhamady AA, Mariey Samah. A, Elewa TA. Assessment of water deficiency tolerance indices and their relation with ISSR markers in Barley (Hordeum vulgare L.). Cu Sci Inte. 2019;8:83.
Shata SM, Said WM, Abdel-Tawab FM, Kamal LM. Morphological and quantitative traits of phylogenetic relationships of some Barley (Hordeum vulgare L.) accessions in Egypt. Journal of Scientific Research in Science. 2021;38:16.
Mohamed AH, Omar AA, Attya AM, Elashtokhy MMA, Zayed EM, Rizk RM. Morphological and molecular characterization of some Egyptian six-rowed barley (Hordeum vulgare L.). Plants. 2021;10:2527
ISTA, International Seed Testing Association. International rules for seed testing. Zurich: ISTA; 2008.
Association of Official Analytical Chemists (AOAC)"Official method of analysis" (17th ed.). Gaithersburg, MD, USA. 2000;967: 21.
Bouslama M, Schapaugh WT. Evaluation of three screening techniques for heat and drought tolerance. Crop Science. 1984;24:933–937.
Golestani Araghi S, Assad M. Evaluation of four screening techniques for drought resistance and their relationship to yield reduction ratio in wheat. Euphytica. 1998;103: 293:299 .
Fischer RA, Maurer R. Drought resistance in spring wheat cultivars. Aust. J. Agric. Res. 1978;29:897.
Fernandez GCJ. Effective selection criteria for assessing stress tolerance. Proceedings of the International Symposium on Adaptation of Vegetables and Other Food Crops in Temperature and Water Stress, Publication, Tainan, Taiwan; 1992.
Rosielle AA, Hamblin J. Theoretical aspects of selection for yield in stress and nonstress environment. Crop Sci. 1981;21:943.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Focus. 1990;12:13-15.
SAS. Base SAS® 9.3 Procedures Guide. SAS Cary NC: USA; 2011.
Fisher RA. The design of experiments. Oliver & Boyd, Edinburgh; 1935.
Yan W, Rajcan I. Biplot analysis of test sites and trait relations of soybean in Ontario. Crop Sci. 2002;42:11-20.
Metsalu T, Vilo J. ClustVis: A web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap. Nucleic Acids Res. 2015;43:566.
Anderson JA, Churchill GA, Autrique, Tanksley JE, SD, ME. Optimizing parental selection for genetic linkage maps. Genome. 1993;36:181
Amiryousefi A, Hyvönen J, Poczai P. iMEC: Online marker efficiency calculator. Appl. Plant Sci. 2018;6:01159
Hammer ØD. Harper AT, Ryan PD. Paleontological statistics software package for education and data analysis, Palaeontologia Electronica. 2001;4:1.
Younis A, Ramzan F, Ramzan Y, Zulfiqar F, Ahsan M, Lim KB. Molecular markers improve abiotic stress tolerance crops: A review. Plants. 2020;9:1374.
Mwando E, Angessa TT, Han Y, Chengdao L. Salinity tolerance in Barley during germination—homologs and potential genes. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 2020;21:93.
Rajeswari S, Sood N, Gokhale ST, Subramanian R. Assessing salt-stress tolerance in Barley. Universitas Scientiarum. 2019;24 :91.
Saleh A, Abd El-Hamid H, Shaddad MA, El-badry N. Assessment the growth and some chemical contents of three Barley cultivars under salt stress. Journal of Environmental Sciences. 2017;46:227.
Nadeem M, Tariq MN, Amjad M, Sajjad M, Akram M, Imran M, Kulikov D. Salinity induced changes in the nutritional quality of bread wheat (Triticum aestivum L.) genotypes. Journal of Agricultural Science. 2020; 42: 1.
Rasheed A, Feng Q, Adil MF, Ahsan M, Han Z, Zeng F, Shamsi IH. Comparative study on the physio-biochemical responses of spring and winter barley genotypes under vernalized and greenhouse conditions. Agronomy. 2022;12:339.
Pour Aboughadareh A, Sanjani S, Nikkhah Chamanabad H, Mohammad Mehrvar, Asadi A, Amini A. Identifcation of salt tolerant Barley genotypes using multiple traits index and yield performance at the early growth and maturity stages. Bull Natl Res Cent. 2021;45:117.
Hilmarsson HS, Rio S, Sánchez JIy. Genotype by environment interaction analysis of agronomic spring barley traits in iceland using AMMI, factorial regression model and linear mixed model. Agronomy 2021;11: 499.
Malik WA, Forkman J, Piepho HP. Testing multiplicative terms in AMMI and GGE models for multienvironment trials with replicates. Theoretical and Applied Genetics. 2019;132:2087.
Khatab Ismael A, Mareiy A. Samah Development of agronomical and molecular genetic markers associated with salt stress tolerance in some barley genotypes. Current Research Journal of Biological Sciences. 2013; 5:198-204.
Heiba SAA, Haiba AAA, Abdel- Rahman HM. Determination of genetic markers in some Egyptian varieties of wheat and barley under salt and drought stresses. Asian Journal of Crop Science. 2019;11:59-70.
Bashandy T. Assessment of molecular diversity and tolerance of increased salinity levels in irrigation of Barley seedlings. J. Agric. Chem. and Biotechn., Mansoura Univ. 2016;9:257 -261.
Al Lawati, Ali H, Nadaf, Saleem K, AlSaady, Nadiya A, Al Hinai, Saleh A, Almamari, Almandhar R, Al Maawali, Abdulaziz A. Genetic diversity of Omani Barley (Hordeum vulgare L.) germplasm" Open Agriculture, 2021;6:628.
Waqas Manzoor Bhutta. Identification of salinity resistance wheat (Triticum aestivum L.) genotypes by SSR markers. Biomed J Sci & Tech Res. 2021;35:202.
FAO. Land and Plant Nutrition Management Services; 2008. Available:http://www.fao.org/agb/agl/agll/spush/
Egyptian Environmental Affairs Agency (EEAA). Egypt National 3rd communication Under UNFCCC. Cairo: Egyptian Environmental Affairs Agency (EEAA) (2016).
FAOSTAT, D. Food and Agriculture Organization of the United Nations. Statistical Database. FAOSTAT. 2021;XVII. Available:http://www.fao.org/faostat/en/#home (accessed on 12 January 2021).
Mariey Samah A, Amany M. Mohamed and Heba G. Ali. Effect of salinity stress on physiological and biochemical traits of Barley cultivars. International Journal of Environment. 2018a;7: 65-77.
Hammami Z, Qureshi A, Sahli A, Gauffreteau A, Chamekh Z, Ben Azaiez Fatma E, Ayadi S, Trifa Y. Modeling the effects of irrigation water salinity on growth, yield and water productivity of barley in three contrasted environments. Agronomy. 2020;10:1459.
Awad A, Odat N, Abu-Romman S, Hasan M, Al-Tawaha AR. Effect of salinity on germination and root growth of Jordanian Barley. Journal of Ecological Engineering. 2021;22:41.
Mariey, Samah A, Khedr Rania A, Zayed, B. A., and Elakhdar A. Genetic Variability among Egyptian Barley varieties for agro- morphological traits under saline soil condition .Egypt. J. Plant Breed. 2017;21:577.
Moustafa, Ehab SA. Assessment of genetic variations and interrelationships among agronomic traits in advanced breeding Barley lines under salinity condition. Egyptian J. Desert Res. 2021;71:1-22.
Mansour E, Moustafa S, Mohamed IE, Abdul-Hamid, Salwa MAI. Ash-shormillesy, Abdel-Rahman MA. Merwad, Hany AW, Ernesto I. Field responses of Barley genotypes across a salinity gradient in an arid mediterranean environment. Agricultural Water Management. 2021;258:107-206.
Jamshidi A, Javanmard HR. Evaluation of Barley (Hordeum vulgare L.) genotypes for salinity tolerance under field conditions using the stress indices. Ain Shams Eng J. 2018;9: 2093:2099.
Mariey Samah A, Ahmed KR, Agwa AME, Farid Mona. A, Serag AM. Biochemical and molecular genetic markers associated with salt stress tolerance in Egyptian Barley cultivars. Egypt. J. Plant Breed. 2019;23:183.
Allel D, BenAma A, Badri r, M, Abdelly C. Evaluation of salinity tolerance indices in North African Barley accessions at reproductive stage. Czech J. Genet. Plant Breed. 2019;55:61.
Moustafa ESA, Ali MMA, Kamara MM, Awad MF, Hassanin AA, Mansour E. Field screening of wheat advanced lines for salinity tolerance. Agronomy. 2021;11:281.
Oubaidou R, Hentour S, Houasli C, Aboutayeb, R, El Goumi Y, El Maaiden E, Fakiri M. Evaluating salt tolerance in doubled haploid Barley lines using a multivariable screening approach. Biocatalysis and Agricultural Biotechnology. 2021;35:102060.
Mariey Samah A, Mohamed Eman N, Ghareeb Zeinab E, Abo Zaher Engy S. Genetic diversity of Egyptian Barley using agro–physiological Traits, grain quality and molecular markers. Current Science International. 2021;10:58.
Godwin ID, Aitken EA, Smith LW. Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis. 1997;18:1524.
Reddy MP, Sarla N, Siddiq E. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica. 2002; 128:9–17.
Mareiy A. Samah, Mona A. Farid and karima A. R) Morphological and Molecular Characterization of Some Egyptian Barley Cultivars under Calcareous Soil conditions. Middle East Journal of Agriculture Research. 2018b , 07: 408- 420
Velicevici G, Madoşă E, Ciulca S, Camen D, Ciulca A, Petolescu C, Malaescu M, , Nistor E, Beinsan C. Analysis of genetic diversity in Barley cultivars using ISSR markers. Journal of Horticulture, Forestry and Biotechnology. 2018;22:19.
Khatab IA, El-Mouhamady AA, Mariey Samah. A, Elewa TA. Assessment of water deficiency tolerance indices and their relation with ISSR markers in Barley (Hordeum vulgare L.). Cu Sci Inte. 2019;8:83.
Shata SM, Said WM, Abdel-Tawab FM, Kamal LM. Morphological and quantitative traits of phylogenetic relationships of some Barley (Hordeum vulgare L.) accessions in Egypt. Journal of Scientific Research in Science. 2021;38:16.
Mohamed AH, Omar AA, Attya AM, Elashtokhy MMA, Zayed EM, Rizk RM. Morphological and molecular characterization of some Egyptian six-rowed barley (Hordeum vulgare L.). Plants. 2021;10:2527
ISTA, International Seed Testing Association. International rules for seed testing. Zurich: ISTA; 2008.
Association of Official Analytical Chemists (AOAC)"Official method of analysis" (17th ed.). Gaithersburg, MD, USA. 2000;967: 21.
Bouslama M, Schapaugh WT. Evaluation of three screening techniques for heat and drought tolerance. Crop Science. 1984;24:933–937.
Golestani Araghi S, Assad M. Evaluation of four screening techniques for drought resistance and their relationship to yield reduction ratio in wheat. Euphytica. 1998;103: 293:299 .
Fischer RA, Maurer R. Drought resistance in spring wheat cultivars. Aust. J. Agric. Res. 1978;29:897.
Fernandez GCJ. Effective selection criteria for assessing stress tolerance. Proceedings of the International Symposium on Adaptation of Vegetables and Other Food Crops in Temperature and Water Stress, Publication, Tainan, Taiwan; 1992.
Rosielle AA, Hamblin J. Theoretical aspects of selection for yield in stress and nonstress environment. Crop Sci. 1981;21:943.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Focus. 1990;12:13-15.
SAS. Base SAS® 9.3 Procedures Guide. SAS Cary NC: USA; 2011.
Fisher RA. The design of experiments. Oliver & Boyd, Edinburgh; 1935.
Yan W, Rajcan I. Biplot analysis of test sites and trait relations of soybean in Ontario. Crop Sci. 2002;42:11-20.
Metsalu T, Vilo J. ClustVis: A web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap. Nucleic Acids Res. 2015;43:566.
Anderson JA, Churchill GA, Autrique, Tanksley JE, SD, ME. Optimizing parental selection for genetic linkage maps. Genome. 1993;36:181
Amiryousefi A, Hyvönen J, Poczai P. iMEC: Online marker efficiency calculator. Appl. Plant Sci. 2018;6:01159
Hammer ØD. Harper AT, Ryan PD. Paleontological statistics software package for education and data analysis, Palaeontologia Electronica. 2001;4:1.
Younis A, Ramzan F, Ramzan Y, Zulfiqar F, Ahsan M, Lim KB. Molecular markers improve abiotic stress tolerance crops: A review. Plants. 2020;9:1374.
Mwando E, Angessa TT, Han Y, Chengdao L. Salinity tolerance in Barley during germination—homologs and potential genes. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 2020;21:93.
Rajeswari S, Sood N, Gokhale ST, Subramanian R. Assessing salt-stress tolerance in Barley. Universitas Scientiarum. 2019;24 :91.
Saleh A, Abd El-Hamid H, Shaddad MA, El-badry N. Assessment the growth and some chemical contents of three Barley cultivars under salt stress. Journal of Environmental Sciences. 2017;46:227.
Nadeem M, Tariq MN, Amjad M, Sajjad M, Akram M, Imran M, Kulikov D. Salinity induced changes in the nutritional quality of bread wheat (Triticum aestivum L.) genotypes. Journal of Agricultural Science. 2020; 42: 1.
Rasheed A, Feng Q, Adil MF, Ahsan M, Han Z, Zeng F, Shamsi IH. Comparative study on the physio-biochemical responses of spring and winter barley genotypes under vernalized and greenhouse conditions. Agronomy. 2022;12:339.
Pour Aboughadareh A, Sanjani S, Nikkhah Chamanabad H, Mohammad Mehrvar, Asadi A, Amini A. Identifcation of salt tolerant Barley genotypes using multiple traits index and yield performance at the early growth and maturity stages. Bull Natl Res Cent. 2021;45:117.
Hilmarsson HS, Rio S, Sánchez JIy. Genotype by environment interaction analysis of agronomic spring barley traits in iceland using AMMI, factorial regression model and linear mixed model. Agronomy 2021;11: 499.
Malik WA, Forkman J, Piepho HP. Testing multiplicative terms in AMMI and GGE models for multienvironment trials with replicates. Theoretical and Applied Genetics. 2019;132:2087.
Khatab Ismael A, Mareiy A. Samah Development of agronomical and molecular genetic markers associated with salt stress tolerance in some barley genotypes. Current Research Journal of Biological Sciences. 2013; 5:198-204.
Heiba SAA, Haiba AAA, Abdel- Rahman HM. Determination of genetic markers in some Egyptian varieties of wheat and barley under salt and drought stresses. Asian Journal of Crop Science. 2019;11:59-70.
Bashandy T. Assessment of molecular diversity and tolerance of increased salinity levels in irrigation of Barley seedlings. J. Agric. Chem. and Biotechn., Mansoura Univ. 2016;9:257 -261.
Al Lawati, Ali H, Nadaf, Saleem K, AlSaady, Nadiya A, Al Hinai, Saleh A, Almamari, Almandhar R, Al Maawali, Abdulaziz A. Genetic diversity of Omani Barley (Hordeum vulgare L.) germplasm" Open Agriculture, 2021;6:628.
-
Abstract View: 322 times
PDF Download: 2 times
Download Statistics
Downloads
Download data is not yet available.
Current Issue
Subscription
Login to access subscriber-only resources.