PATTERN OF HEPATIC ENZYMES PROFILE IN SICKLE CELL DISEASE PATIENTS ATTENDING MADONNA UNIVERSITY TEACHING HOSPITAL (MUTH)

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Published: 2022-12-31

Page: 34-38


NNODIM JOHNKENNEDY *

Department of Medical Laboratory Science, Imo State University, Owerri, Nigeria.

NWOKEJI CHIBUDO ODERA

Department of Medical Laboratory Science, Madonna University, Elele Rivers, Nigeria.

NWOKEJI CALISTUS MUODEBE

Department of Medical Laboratory Science, Madonna University, Elele Rivers, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Sickle cell anaemia   is  a  lifelong  blood  disorder characterized  by  red  blood  cells  that  assume  an abnormal,  rigid,  sickle  shape. The sickling occurs  because  of  a mutation in the haemoglobin gene. This study, is aimed at evaluating liver enzymes in Sickle cell anaemia. Before the commencement of the study, ethical approval was obtained from the Madonna University teaching hospital Ethical Committee. The study consists of 233 subjects divided into two (2) groups. Ninety (90) subjects with Hemoglobin Genotype HbAA and one Hundred and fourty three (143) subjects with the Genotype HbSS. Cellulose acetate alkaline hemoglobin electrophoresis technique was used in determination of hemoglobin genotype. Reitman-frankel method was used in in-vitro determination of ALT and AST activities. Phenolphthalein Monophosphate method    was used in in-vitro determination of ALP activity. The data obtained was analysed using Statistical Package of Social Sciences (SPSS) Version 26. There was a significant difference in AST levels (IU/l) of HbAA (22.46±2.51) when compared to HbSS (72.03±25.94), t(126) = 10.07, p. value = 0.000. There was also a significant difference in ALP levels (IU/l) of HbAA (19.54±3.34) when compared to HbSS(70.25±17.65), t(126) = 15.09, p. value = 0.000. A significant difference, was also observed in ALT levels (IU/l) of HbAA(16.53±2.51) when compared to HbSS(64.50±27.17) t(103) = 17.44, p. value = 0.000. Patients with sickle cell disease can develop liver disease as a result of intrahepatic sickling of erythrocytes, viral hepatitis and iron overload secondary to multiple blood transfusions, and gallstone disease as a result of chronic hemolysis.

 

Keywords: Pattern, hepatic enzymes, sickle cell disease, patients, Madonna University Teaching Hospital


How to Cite

JOHNKENNEDY, N., ODERA, N. C., & MUODEBE, N. C. (2022). PATTERN OF HEPATIC ENZYMES PROFILE IN SICKLE CELL DISEASE PATIENTS ATTENDING MADONNA UNIVERSITY TEACHING HOSPITAL (MUTH). Asian Journal of Research in Biology, 5(1), 34–38. Retrieved from https://ikprress.org/index.php/AJRiB/article/view/8120

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