Klebsiella pneumonia Carbapenemase and New Delhi Mettallo-1 Genes: Molecular Characterization and Detection from Clinical Isolates in Madonna University Teaching Hospital Elele

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Published: 2023-01-10

Page: 1-14


Dunga Kingsley Excel

Department of Medical Laboratory Science, Rhema University, Aba, Nigeria.

Ofoegbu Nnamdi Jude

Department of Medical Laboratory Science, Rhema University, Aba, Nigeria.

Y. M. Tatfeng

Department of Medical Laboratory Science, Niger Delta University Amassom, Bayesa State, Nigeria.

J. O. Njoku

Department of Public Health, Federal University of Technology Owerri, Nigeria.

Okoro Chinyere Ihuarulam

Department of Microbiology/Parasitology, Federal Medical Centre, Owerri, Nigeria.

C. N. Ohalette

Department of Microbiology, Imo State University Owerri, Nigeria.

Nnodim Johnkennedy *

Department of Medical Laboratory Science, Imo State University Owerri, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Gram-negative, non-motile, encapsulated, lactose-fermenting, facultatively anaerobic, rod-shaped Klebsiella pneumonia is a bacterium. On MacConkey agar, it manifests as a mucoid lactose fermenter. Despite being a typical component of the flora in the intestines, skin, and mouth. Multidrug resistance (MDR) Enterobacteriaceae infections are a significant global source of morbidity and mortality. The most popular drug used in this therapy is carbapenem. The emergence of these agents' resistance has raised severe health concerns throughout the world. An enzyme called Klebsiella pneumonia carbapenemase (KPC) and New Delhi metallo-beta-lactamase 1 (NDM-1) makes bacteria resistant to a variety of beta-lactam drugs. The clinical specimen was used for the molecular detection of the NDM-1 and KPC betalactamase genes in Klebsiella pneumonia species. Gram staining and biochemical testing were used to phenotypically identify the isolates after they had been grown. Using the boiling technique and Nanodrop, DNA was extracted, quantified, and purified. There were 192 isolates in all, of which 18 (or 9.4%) were found to be multidrug resistant. The remaining isolates came from urine, wounds, high vaginal swabs, urine, and urethra swabs, respectively. During the megablast search for extremely similar sequences from the NCBI non redundant nucleotide (nr/nt) database, the isolated 16srRNA sequence produced a perfect match. The evolutionary distances calculated using the Jukes-Cantor method were consistent with the phylogenetic placement of isolates K1, K2, K3, and K5 within the Klebsiella sp. They also revealed a closer relationship between K4 and Enterobacter hormaechi subsp. xiangfangensis strain BHW6 and Klebsiella pneumonia sihong775 2 than other Klebsiella sp. The specimen and the presence of betalactam klebsiella pnuemoniae differed significantly (p 0.05). Genotypical analysis of the samples revealed the presence of KPC6 (33.3%) and NDM-14(22.2%). Therefore, understanding the molecular epidemiology and drug resistance mechanisms of carbapenemase-producing K. pneumonia can help with clinical therapy and infection prevention strategies for patients.

Keywords: Klebsiella pneumonia, clinical samples, molecular characterization, detection kpc and NDM-1 genes


How to Cite

Excel, D. K., Jude, O. N., Tatfeng, Y. M., Njoku, J. O., Ihuarulam, O. C., Ohalette, C. N., & Johnkennedy, N. (2023). Klebsiella pneumonia Carbapenemase and New Delhi Mettallo-1 Genes: Molecular Characterization and Detection from Clinical Isolates in Madonna University Teaching Hospital Elele. Asian Journal of Research in Biology, 6(1), 1–14. Retrieved from https://ikprress.org/index.php/AJRiB/article/view/8141

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