CHARACTERIZATION OF CONFORMATIONAL CHANGES OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN BULK SOLUTION AT ACID AND BASE pH USING FLORESCENCE MEASUREMENTS TECHNIQUE

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Published: 2017-10-07

Page: 16-23


ABUBAKER K. ELAYATT *

Department of Chemical Engineering, Faculty of Engineering, University of Sirte, Libya.

EL FATEH BELKASEM

Department of Chemistry, Faculty of Science, University of Benghazi, Libya.

ABDELQADER IMRAGAA

Department of Chemistry, Faculty of Science, University of Benghazi, Libya.

KHALED EDBEY

Department of Chemistry, Faculty of Science, University of Benghazi, Libya.

*Author to whom correspondence should be addressed.


Abstract

The conformational changes of extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different pH values in solution where studied using fluorescence spectroscopy. The EPS was labeled using 8-aminopyrene-1,3,6-trisulfonic acid, tri sodium salt (APTS). The interactions between APTS and EPS surface in dilute aqueous solution were monitored using fluorescence spectroscopy. Steady state, excited state and time-resolved anisotropy measurements (TRAMS) were used to provide information about the changes in conformational behavior of the APTS as they bind to EPS surfaces.

Keywords: Extracellular polymeric substances, fluorescence steady state measurement, Steady State technique, time resolved anisotropy measurements


How to Cite

ELAYATT, A. K., BELKASEM, E. F., IMRAGAA, A., & EDBEY, K. (2017). CHARACTERIZATION OF CONFORMATIONAL CHANGES OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN BULK SOLUTION AT ACID AND BASE pH USING FLORESCENCE MEASUREMENTS TECHNIQUE. Journal of Applied Physical Science International, 9(1), 16–23. Retrieved from https://ikprress.org/index.php/JAPSI/article/view/3025

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