CRESCEIAALATA FLOWER EXTRACT AS REDUCING CATALYST FOR GREEN SYNTHESIS OF Au/Ag BIMETALLIC NANO MEDICINE AND ITS ANTIBACTERIAL ACTIVITIES
C. JEEVARATHINAM *
Department of Chemistry, Arignar Anna Govt. Arts & Science College, Karaikal, India.
J. SAMU SOLOMON
Department of Chemistry, T.B.M.L College, Porayar, India.
G. V. PANDIAN
Department of Chemistry, T.B.M.L College, Porayar, India.
*Author to whom correspondence should be addressed.
Abstract
The green fabrication of metal nanoparticles by using botanical extracts is significantly important for increasing research attention in nanotechnology, since it does not require high energy inputs or the production of highly toxic chemicalbyproducts. Here, silver (Ag), gold (Au) and their bimetallic (Ag/Au) nanoparticles (NPs) were green synthesized using the Crescentiaalata aqueous flower extract. Metal NPs were studied by spectroscopic (UV–visible spectroscopy, fluorescence spectroscopy, FT-IR spectroscopy, XRD and EDX) and microscopic (AFM and TEM) analysis. AFM and TEM showed that Ag and Au NPs had triangular and spherical morphologies, with an average size of 20 nm. Bimetallic Ag/Au NPs showed spherical shapes with an average size of 10 nm. Ag and Ag/Au bimetallic NPs showed high antibacterial and antibiofilm activities towards Gram-positive and Gram-negative bacteria. Overall, the proposed synthesis route of Ag, Au and Ag/Au bimetallic NPs can be exploited by the pharmaceutical industry to develop drugs effective in the fight against microbic infections.
Keywords: Biofabrication, biosafety, Crescentiaalata, nanotechnology, surfaceplasmon resonance
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Björnmalm M, Faria M., Caruso F. Increasing the impact of materials in and beyond bio-nano science. J. Am. Chem. Soc. 2016;138:13449–13456.Lin PC, Lin S, Wang PC, Sridhar R. Techniques for physicochemical characteriza-tion of nanomaterials. Biotechnol. Adv. 2014; 3:711–726.
Syed B, Bisht N, Bhat PS, Karthik RN, Prasad A, Dhananjaya BL, Satish S, Prasad H, Nagendra Prasad MN. Phytogenic synthesis of nanoparticles from Rhizophora mangle and their bactericidal potential with DNA damage activity. Nano-Structures & Nano-Objects. 2017a;10:112–115.
Kavitha KS, Baker S, Rakshith D, Kavitha HU, Rao HCY, Harini BP, Satish S. Plants as green source towards synthesis of nanoparticles. Int. Res. J. Biol. Sci. 2013;2:66–76.
Baker S, Harini BP, Rakshith D, Satish S. Plants: Emerging as nanofactories towards facile route in synthesis of nanoparticles. Bioimpacts. 2013;3:111–117.
Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: Synthesis characterization, properties, applications and therapeutic approaches. Int. J. Mol. Sci. 2016;17:1534.
Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect. Med. Chem. 2014;6:25–64.
Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol. Mol. Biol. Rev. 2010;74:417–433.
Ventola CL. The antibiotic resistance crisis: Part 1: causes and threats. Pharm. Ther. 2015; 40:277–283.
Iravani S. Green synthesis of metal nanoparticles using plants. Green. Chem. 2011; 13:2638–2650.
Baker S, Satish S. Endophytes: Toward a vision in synthesis of nanoparticle for future therapeutic agents. Int.J. Bio-Inorg. Hybrid Nanomater. 2012;2:67–77.
Syed B, Nagendra Prasad MN, Dhananjaya BL, Mohan Kumar K, Yallappa S, Satish S. Synthesis of silver nanoparticles by endosymbiont Pseudomonas fluorescens CA 417 and their bactericidal activity. Enzyme Microb. Technol. 2016;95:128–136.
Baker S, Nagendra-Prasad MN, Satish S. Synthesis and characterization of silver nanobactericides produced by Aneurinibacillus migulanus 141, a novel endophyte inhabiting Mimosa pudica L. Arab. J. Chem; 2016.
Available:https://doi.org/10.1016/j. arabjc.2016.01.005
Elavazhagan T, Arunachalam KD. Memecylonedule flower extract mediated green synthesis of silver and gold nanoparticles. Int. J. Nanomed. 2011;6:1265–1278.
Keat CL, Aziz A, Eid AM, Elmarzugi NA. Biosynthesis of nanoparticles and silver nano-particles. Bioresour. Bioprocess. 2015;2: 47.
Qian Y, Huimei Yu, He Dan, Yang Hui, Wang, Wanting Wan, Xue Wang Li. Biosynthesis of silver nanoparticles by the endophytic fungus Epicoccum nigrum and their activity against pathogenic fungi. Bioprocess Biosyst. Eng. 2013;36:1613– 1619.
Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42:321–324.
Yong Song J, Kim-Beom S. Rapid biological synthesis of silver nanoparticles using plant flower extracts. Bioprocess. Biosyst. Eng. 2009;32:79–84.
Sheny DS, Mathew J, Philip D. Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried flower of Anacardiumoccidentale. Spectrochim. Acta. 2011; 79(Part A):254–262.
Syed B, Nagendra Prasad MN, Mohan Kumar K, Dhananjaya BL, Satish S. Endo-symbiont mediated synthesis of gold nanobactericides and their activity against human pathogenic bacteria. Environ. Toxicol. Pharmacol. 2017b; 52:143–149.
Vanitha V, Umadevi KJ, Vijayalakshmi K. Determination of bioactive components of Crescentia alata L. flower by GC-MS analysis. Int. J. Pharm. Sci. Drug. Res. 2011;4:309–312.
Tamuly C, Hazarika M, Ch. Borah S, Das MR, Boruah MP. In situ biosynthesis of Ag, Au and bimetallic nanoparticles using Piper pedicellatum C. DC: Green chemistry approach. Colloids Surf. B. 2013;102:627–634.
Urbaniak-Domalaga W. The use of spectrometric technique FTIR-ATR to examine polymer surface. Advance Aspects of Spectroscopy. Rijeka (Croatia): Intech Open Science; 2012.
Fan M, Dai D, Huang B. Fourier transform infrared spectroscopy for natural fibres. In: Salih, Salih Mohammed (Ed.), Fourier Transform – Materials Analysis. InTech; 2012.
Available:https://doi.org/10.5772/35482.
Devi TSR, Gayathri S. FTIR and FT-Raman spectral analysis of paclitaxel drugs. Int. J. Pharm. Sci. Rev. Res. 2010;2:106–110.
Dauthal P, Mukhopadhyay M. Noble metal nanoparticles: Plant-mediated synthesis, mechanistic aspects of synthesis and applications. Ind. Eng. Chem. Res. 2016;55: 9557–9577.
Banerjee M, Sharma S, Chattopadhyay A, Ghosh SS. Enhanced antibacterial activity of bimetallic gold-silver core-shell nanoparticles at low silver concentration. Nanoscale. 2011;3: 5120–5125.
Baker S, Azmath P, Satish S. Biogenic nanoparticles bearing antibacterial activity and their synergistic effect with broad spectrum antibiotics: Emerging strategy to combat drug resistant pathogens, Saudi. Pharm. J; 2015.
DOI: 10.1016/j. jsps.2015.06.011
Awwad AM, Salem NM, Abdeen AO. Green synthesis of silver nanoparticles using carob flower extract and its antibacterial activity. Int. J. Indus. Chem. 2013;1:1–6.
Dimitratos N, Lopez-Sanchez JA, Anthonykutty JM, Brett G, Carley AF, Tiruvalam RC, Herzing AA, Kiely CJ, Knight DW, Hutchings GJ. Physical chemistry chemical physics. 2009;11:952-4961
Klaus TJR, Olsson E, Granqvist C. Gr. ProcNatlAcadSci USA. 1999;96:13611-13614.
Zhao Y, Nalwa HS. Nanotoxicology: Interactions of nanomaterials with biological systems. American Scientific Publishers, Los Angeles. 2007;263.
Singh S, Nalwa HS. Nanotechnology and health safety– toxicity and risk assessments of nanostructured materials on human health. J. Nanosci. Nanotechnol. 2007;7:3048-264.
Khan AK, Rashid R, Murtaza G, Zahra A. Gold nanoparticles: synthesis and applications in drug delivery. Tropical Journal of Pharmaceutical Research. 2014;13(7):1169-1177.