Phytochemical and Cytotoxicity of Acetone Extract of Rosmarinus officinalis L. (Rosemary) Via BSLT
Felycitae Ekalaya Appa *
Pharmacy Department, Cenderawasih University, Papua, Indonesia.
Septaria Yolan Kalalinggi
Chemistry Department, Palangkaraya University, Central Kalimantan, Indonesia.
Yunita Pare Rombe
Chemistry Education Department, Papua University, West Papua, Indonesia.
Nur Fadilah Bakri
Pharmacy Department, Cenderawasih University, Papua, Indonesia.
Yohana Krisostoma Anduk Mbulang
Pharmacy Department, Cenderawasih University, Papua, Indonesia.
Nada Pertiwi Papriani
Chemistry Department, Cenderawasih University, Papua, Indonesia.
Rafsanjany Ramadan
Chemistry Department, Papua University, West Papua, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
Background: Since ancient times, medicinal plants have been extensively utilized for the treatment of various diseases and the promotion of overall health and well-being. The application of herbal plants in traditional and modern medicine continues to gain widespread attention due to their therapeutic potential. One such plant is Rosmarinus officinalis L., an aromatic herb native to the Mediterranean region that has recently begun to be cultivated in Indonesia. This plant is known to contain a wide range of secondary metabolites with diverse biological activities, attracting considerable interest among researchers. However, studies focusing on the semi-polar extracts of rosemary remain limited. Therefore, preliminary investigations on the semi-polar extracts of rosemary are necessary to further explore their potential biological properties and applications.
Aims: The objective of this study is to evaluate the phytochemical constituents and assess the cytotoxicity level of the acetone extract of Rosmarinus officinalis L. (rosemary) via BSLT.
Methodology: The stages of working on this research include extraction, evaporation, and approval. Reserve composition analysis includes phytochemical tests, FTIR, and GCMS analysis.
Results: The compounds rosemary acetone extract are alkaloids, flavonoids, phenols, steroids, and terpenoids. The Value of the cytotoxic test with probit analysis showed LC50 0.061 µg/mL. The major components are sakuretin and isocornasol based on GCMS data.
Conclusion: Cytotoxic test results on rosemary acetone extract are toxic, so that potentially as an anticancer, these have a positive assessment of the analysis of collections that contain secondary metabolites.
Keywords: Acetone extract, BSLT, cytotoxicity, phytochemical, rosemary