ANNEXIN A1 AND FORMYL PEPTIDE RECEPTOR IN GESTATIONAL TROPHOBLASTIC DISEASES: FOCUS ON PROLIFERATION AND APOPTOSIS
MARYSTELA FÁVERO DE OLIVEIRA CARDOSO
Department of Biology, Instituto de Biociências, Letras e Ciências Exatas, São Paulo State University (UNESP), Rua Cristovão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brazil and From the Post-Graduation in Structural and Functional Biology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 740, São Paulo, 04023-900, Brazil
ANA PAULA GIROL
Department of Biology, Instituto de Biociências, Letras e Ciências Exatas, São Paulo State University (UNESP), Rua Cristovão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brazil and Department of Physical and Biological Sciences, Padre Albino School of Medicine (FIPA), Rua dos Estudantes, 225, Catanduva, SP, 15.809-144, Brazil
ELOISA AMÁLIA VIEIRA FERRO
Departament of Histology and Cell Biology, Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Avenida Pará , 1720, Uberlândia, MG, 38405320, Brazil
SONIA MARIA OLIANI *
Department of Biology, Instituto de Biociências, Letras e Ciências Exatas, São Paulo State University (UNESP), Rua Cristovão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brazil and From the Post-Graduation in Structural and Functional Biology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 740, São Paulo, 04023-900, Brazil
*Author to whom correspondence should be addressed.
Abstract
Introduction: Due to the lack of studies on annexin A1 (ANXA1) in gestational trophoblastic disease (GTD), we evaluated the expression and possible role of this molecule in chorionic villi of normal placentas, but also in complete and partial hydatidiform moles (CHM and PHM).
Methods: Immunohistochemical and densitometric analyses were performed to verify the expressions of ANXA1, formyl peptide receptors (FPR1 and FPR2) and apoptosis in first trimester and full-term placentas, as well as in the CHM and PHM.
Results: We detected increased expression of ANXA1 in the cytotrophoblastic hyperplasia found in the hydropic villi of CHM and PHM. There was no immunoreactivity for FPR2. However, we found specific FPR1 expression in the same regions where the ANXA1 was observed, with higher expression in the syncytiotrophoblast and the syncytial knots of the CHM and PHM. The apoptosis index was similar to those observed for FPR1.
Conclusions: Taken together, the results suggest that ANXA1 may be involved in the cell proliferation and apoptosis processes in the normal placentas and in GTDs. Furthermore, the data indicate that the actions of ANXA1 are specifically mediated by FPR1 and may allow further studies of the ANXA1 in both normal placentas and GTDs.
Keywords: Hydatidiform moles, annexin A1, formyl peptide receptor, apoptosis