LABORATORY DIAGNOSIS OF MALARIA: STUDY OF CONCORDANCE AMONG QUANTITATIVE BUFFY COAT, PERIPHERAL SMEAR AND ANTIGEN DETECTION TESTS

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Published: 2016-03-13

Page: 100-106


VIDYAVATHI B. CHITHARAGI

Department of Microbiology, JSS Medical College, Mysore, India

A. RENUKA

Department of Microbiology, Kasturba Medical College, Manipal, India

G. S. AJANTHA

Department of Microbiology, SDM Medical College, Dharwad, India

U. AMRUTHKISHAN

Department of Microbiology, Subbaiah Institute of Medical Sciences, Shivamogga, India

K. R. PRAVIN CHANDRA

Department of Community Medicine, SDM Medical College, Dharwad, India

R. D. KULKARNI *

Department of Microbiology, SDM Medical College, Dharwad, India

*Author to whom correspondence should be addressed.


Abstract

Background and Objectives: Malaria is a serious, sometimes fatal disease posing a major public health problem in India. Early diagnosis and prompt treatment is the key to minimize the morbidity and mortality due to malaria. Peripheral blood smear examination is the “gold standard” for the diagnosis of malaria. However, microscopy is labor intensive, requires significant skill and time, leading to therapeutic delays. Newer methods such as Quantitative buffy coat (QBC) and Rapid diagnostic tests (RDTs) are being widely used for diagnosis. In the present study, efficacy of peripheral smear (PS) and Antigen detection (Ag) was compared taking QBC as a standard.

Methods: Total of 124 clinically suspected cases of malaria were included in the study. QBC positive 62 samples and age & sex matched QBC negative 62 samples were further evaluated by PS and Ag.

Results: In the present study, the sensitivity, specificity, positive predictive value and negative predictive value of PS were found to be 95.2%, 100%, 100% and 95.4% respectively. Sensitivity and specificity of antigen detection was found to be 93.5% and 100% respectively. Positive predictive value of antigen detection was found to be 100% and negative predictive value was 93.9%.

Interpretation and Conclusion: A test which is rapid and can detect low level of parasitemia should be used for routine diagnosis in endemic areas. Although PS is cost effective it is difficult to interpret by inexperienced microscopists. The sensitivity of RDTs decreases with parasitemia. QBC is a concentration method for direct demonstration of parasite which is rapid, reliable and easy to perform. QBC should be used for routine diagnosis in the laboratories which screen large number of samples.

 

Keywords: QBC, malaria, RDTs, PS, vivax, falciparum


How to Cite

CHITHARAGI, V. B., RENUKA, A., AJANTHA, G. S., AMRUTHKISHAN, U., CHANDRA, K. R. P., & KULKARNI, R. D. (2016). LABORATORY DIAGNOSIS OF MALARIA: STUDY OF CONCORDANCE AMONG QUANTITATIVE BUFFY COAT, PERIPHERAL SMEAR AND ANTIGEN DETECTION TESTS. Journal of International Research in Medical and Pharmaceutical Sciences, 9(3), 100–106. Retrieved from https://ikprress.org/index.php/JIRMEPS/article/view/2852

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