IMMUNO-DISTRIBUTION AND EXPRESSION OF C-CBL PROTO-ONCOGENE IN HUMAN GASTRIC CANCEROUS TISSUES FROM WESTERN ALGERIA

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Published: 2016-06-10

Page: 18-23


RACHIDA SALAH

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria

NORIA HARIR *

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria and Laboratory of Molecular Microbiology, Proteomics and Health, Sidi bel Abbes, Algeria

FERIEL SELLAM

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria

MALIKA AIDOUNI

Department of Pathology, Oran Military Hospital (HMRUO), Algeria

MILOUD MEDJAMIA

Department of Pathology, Oran Military Hospital (HMRUO), Algeria

NESRINE MRABENT

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria

SOUMIA ZEGGAI

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria

MUSTAPHA DIAF

Department of Biology, Djillali Liabes University, Sidi bel Abbes, Algeria

*Author to whom correspondence should be addressed.


Abstract

Background: Gastric cancer is one of the most common causes of cancer death worldwide.

Aim: The aim of our study was to assess the immunohistochemical expression and distribution as well as the status of c-Cbl protein in human gastric cancerous cells.

Materials and Methods: Ninety six paraffin blocks of cancerous gastric tissues sections were collected from the Department of Pathology, of Oran Military Hospital (Western Algeria) for the period of 2006-2015. We performed an immunohistochemical protocol for c-Cbl detection and a statistical analysis for patients’ clinical-pathological parameters.

Results: 96 patients with gastric cancer were included in the current study. Median age of the patients with gastric cancer was 60 years (range, 27-87). There were 70 (72.9 %) males and 26 (27.1%) females; male to female ratio was about 2.7. The most common histological type was moderately differentiated adenocarcinoma 33 cases (34.4%). Our immunohistochemical analysis revealed that c-CBL protein was expressed in gastric cancerous cells but with different of immune-scoring and different immunostaining intensity. The most predominant observed score was score 2 in 20 cases (20.8%) followed by score 4 in 16 cases (16.7%); then score 3, 6 and 7. Concerning immunostaining levels; we reported an intense staining in 17 cases (17.7%); a moderate staining in 31 cases (32.3%); a poor staining in 27 cases (28.1%) and no staining in 21 cases (21.9%).

Conclusion: Our findings revealed that cCbl protein is expressed with important levels in human gastric cancer cells; which could be a good biomarker for gastric cancer diagnosis.

Keywords: c-Cbl protein, gastric cancer, immunohistochimestry, expression


How to Cite

SALAH, R., HARIR, N., SELLAM, F., AIDOUNI, M., MEDJAMIA, M., MRABENT, N., ZEGGAI, S., & DIAF, M. (2016). IMMUNO-DISTRIBUTION AND EXPRESSION OF C-CBL PROTO-ONCOGENE IN HUMAN GASTRIC CANCEROUS TISSUES FROM WESTERN ALGERIA. Journal of International Research in Medical and Pharmaceutical Sciences, 10(1), 18–23. Retrieved from https://ikprress.org/index.php/JIRMEPS/article/view/3078

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