EFFECTS OF Calix[4]arene C-145 ON OVERALL HAEMOSTATIC POTENTIAL OF BLOOD PLASMA In vitro AND In vivo

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Published: 2016-11-26

Page: 146-151


V. O. CHERNYSHENKO *

O.V. Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine

L. V. PIROGOVA

O.V. Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine

V. A. DIDKIVSKYI

Medical Center of Private Higher Educational Institution “Institute of General Practice - Family Medicine”, Kyiv, Ukraine

S. O. CHERENOK

Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Ukraine

V. Е. DOSENKO

O.O. Bohomolets Institute of Physiology, NAS of Ukraine, Ukraine

D. O. PASHEVIN

O.O. Bohomolets Institute of Physiology, NAS of Ukraine, Ukraine

V. I. KALCHENKO

Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Ukraine

E. M. MAKOGONENKO

O.V. Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine

E. V. LUGOVSKOY

O.V. Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine

*Author to whom correspondence should be addressed.


Abstract

Introduction: The overall haemostatic potential (OHP) assay was applied for testing of anticoagulant action of sodium salt of calix[4]arenemethylene-bis-phosphonic acid (C-145) in blood plasma In vitro and In vivo.

Methods: Calix[4]arene C-145 was added to platelet poor plasma or injected intravenously to rabbits in equal concentrations (20 µM), clotting was initiated by APTT-reagent and turbidity change of the mixture was measured in the presence or absence of tPA.

Results: We demonstrated that in vitro 20 μM of C-145 substantially decreased the OHP in 1.5 times and did not influence fibrinolysis. That injection of calix[4]arene in vivo decreased the overall haemostatic potential in 30-10%. The prolongation of half-time of clot lysis was not observed.

Conclusion: Overall haemostatic potential assay allowed us to conclude that coagulation and fibrinolytic branches of haemostasis remain in dynamic balance at C-145 administration. C-145 being taken in the final concentration 20 μM inhibited coagulation potential of blood plasma and did not affect fibrinolysis in vivo.

Keywords: Overall haemostatic potential, calix[4]arene, antithrombotic drugs, fibrinolysis, blood coagulation, haemostasis


How to Cite

CHERNYSHENKO, V. O., PIROGOVA, L. V., DIDKIVSKYI, V. A., CHERENOK, S. O., DOSENKO V. Е., PASHEVIN, D. O., KALCHENKO, V. I., MAKOGONENKO, E. M., & LUGOVSKOY, E. V. (2016). EFFECTS OF Calix[4]arene C-145 ON OVERALL HAEMOSTATIC POTENTIAL OF BLOOD PLASMA In vitro AND In vivo. Journal of International Research in Medical and Pharmaceutical Sciences, 10(3), 146–151. Retrieved from https://ikprress.org/index.php/JIRMEPS/article/view/3248

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