EFFECTS OF D-005, A LIPID EXTRACT FROM COROJO PALM (Acrocomia crispa) FRUITS, ON α 1-ADRENOCEPTORS MEDIATED RESPONSES

Full Article - PDF

Published: 2016-12-09

Page: 163-170


AMBAR OYARZÁBAL YERA

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

VIVIAN MOLINA CUEVAS *

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

ROSA MAS FERREIRO

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

YAZMÍN RAVELO CALZADO

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

YOHANI PÉREZ GUERRA

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

ROXANA SIERRA

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

VICTOR GONZÁLEZ CANAVACIOLO

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

SONIA JIMÉNEZ DESPAIGNE

Centre of Natural Products, National Centre for Scientific Research, Havana City, Cuba

*Author to whom correspondence should be addressed.


Abstract

Enhanced stimulation of bladder and prostatic α1-adrenoreceptors (α1-ADR) triggers lower urinary tract symptoms. Phenylephrine (PHE) induces α1-ADR-mediated smooth muscle contractions In vitro and urodynamic dysfunction in rats. Extracts from saw palmetto and Roystonea regia fruits antagonize α1-ADR responses. Effects of Acrocomia crispa (AC), an endemic Cuban palm, are unexplored. This study investigates the effects of D-005, a lipid extract of AC fruits, on PHE-induced contractions in vas deferens and PHE-induced contractions urodynamic dysfunction in rats. In vitro study: Preparations of rat vas deferens were incubated with Tyrode´s solution (control), tamsulosin 0.1 mg/mL or D-005 (125-500 mg/mL). Contractions were induced with PHE (1 x10-6 - 3x10-4 mol/L) or KCl (50 mmol/L).  In vivo study: Rats were randomized into a negative vehicle control and seven PHE (5 mg/kg)-injected groups: positive control, D-005 (5, 25, 50, 200, 400 mg/kg) and tamsulosin (0.4 mg/kg). Volume voided per micturition (VM) and urinary total volumes (UTV) were measured. Tamsulosin inhibited PHE-induced contractions and did not change KCl-induced contractions. D-005 (250 and 500 μg/mL, not 125 mg/mL) inhibited significantly and markedly contractions to all PHE concentrations and modestly KCl-induced contractions. PHE lowered significantly VM and UTV in positive controls versus the negative control group. Tamsulosin increased VM (78%) and UTV (78%) versus the positive control group. D-005 (25 - 800 mg/kg, not 5 mg/kg) increased significantly and dose dependently VM versus the positive control. All doses increased significantly UTV. In conclusion, D-005 inhibited PHE-induced contractions in vas deferens of rats and ameliorated PHE-induced urodynamic dysfunction in rats, thus antagonizing α1-ADR-mediated responses.

Keywords: D-005, Acrocomia crispa, vas deferens, lower tract urinary symptoms, α1-adrenoreceptors, phenylephrine responses


How to Cite

YERA, A. O., CUEVAS, V. M., FERREIRO, R. M., CALZADO, Y. R., GUERRA, Y. P., SIERRA, R., CANAVACIOLO, V. G., & DESPAIGNE, S. J. (2016). EFFECTS OF D-005, A LIPID EXTRACT FROM COROJO PALM (Acrocomia crispa) FRUITS, ON α 1-ADRENOCEPTORS MEDIATED RESPONSES. Journal of International Research in Medical and Pharmaceutical Sciences, 10(3), 163–170. Retrieved from https://ikprress.org/index.php/JIRMEPS/article/view/3273

Downloads

Download data is not yet available.