ISOQUINOLINE ALKALOID BERBERINE ACTS AS AN INHIBITOR OF OSTEOCLAST DIFFERENTIATION

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Published: 2017-07-05

Page: 131-141


PETYA GANOVA

Department of Immunology, Institute of Microbiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria

VALERIYA GYURKOVSKA

Department of Immunology, Institute of Microbiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria

TSVETELINA DONCHEVA

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Science, 1113, Sofia, Bulgaria

NINA IVANOVSKA *

Department of Immunology, Institute of Microbiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria

*Author to whom correspondence should be addressed.


Abstract

Objective: Berberine, a naturally occurring isoquinoline alkaloid, possesses a wide range of pharmacological activities, including anti-bacterial, anti-fungal, anti-protozoal, anti-inflammatory, anti-diabetes and anti-tumor properties. The purpose of this study was to investigate the effect of berberine on osteoclast differentiation of bone marrow (BM) cells obtained from BALB/c mice with zymosan-induced arthritis (ZIA).

Materials and Methods: Osteoclast precursors were stimulated for 6 days under different schemes and osteoclast formation was assessed by counting the number of tartrate-resistant acid phosphatase (TRAP)-stained multinuclear cells. The expression of receptor activator of nuclear factor κB ligand (RANKL), tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and TRAIL-R2 (DR5) on cultivated cells were determined by flow cytometry.

Results: Berberine inhibited macrophage colony-stimulating factor (M-CSF)+RANKL-induced osteoclast differentiation and also, RANKL-independent osteoclast generation. Anti-osteoclastogenic effect of the alkaloid was related with markedly inhibited secretion of interleukin IL-1 and IL-6, with a decrease of RANKL expression on M-CSF+RANKL-stimulated BM cells, and with a lower expression of DR5.

Conclusion: Our data reveal the mechanisms of blocking osteoclastic differentiation by berberine and it thus holds promise to be further investigated in vivo as a therapeutic or preventive agent for bone-related diseases such as arthritis.

Keywords: Berberine, osteoclasts, cytokines, interleukins


How to Cite

GANOVA, P., GYURKOVSKA, V., DONCHEVA, T., & IVANOVSKA, N. (2017). ISOQUINOLINE ALKALOID BERBERINE ACTS AS AN INHIBITOR OF OSTEOCLAST DIFFERENTIATION. Journal of International Research in Medical and Pharmaceutical Sciences, 11(4), 131–141. Retrieved from https://ikprress.org/index.php/JIRMEPS/article/view/3475

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