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Published: 2021-08-07

Page: 33-39


DM WIMS college of Pharmacy, Wayanad, Kerala, India.


DM WIMS college of Pharmacy, Wayanad, Kerala, India.


DM WIMS college of Pharmacy, Wayanad, Kerala, India.


DM WIMS college of Pharmacy, Wayanad, Kerala, India.

*Author to whom correspondence should be addressed.


Objective: The need of the current study is to explore on the in-vitro anti-inflammatory activity that is the cell membrane stabilizing effect of hydroalcoholic extracts and essential oil by the crude drug Syzygium aromaticum.

Materials and Methods: In this investigation blood sample was withdrawn from healthy human subjects for the preparation of erythrocyte suspension using certain buffers. Then the prepared HRBC suspension was disclosed to essential oil of certain concentration and different graded concentrations of hydroalcoholic extracts from 100 to 1000 μg/mL. The various cytoprotective assays implemented in this research includes HRBC membrane stabilization test, heat induced and hypotonicity induced hemolysis. The standard drug used in this work is Diclofenac Sodium. All the treatment groups were carried out in a triplicate manner and the result obtained is given as Mean ± standard deviation statistically.

Results: It was observed that the in-vitro anti-inflammatory activity of EEZA showed significant result and was effective. The aqueous extracts of the crude drug also showed little anti- inflammatory property. The essential oil also was potent enough to stabilize cell membrane in unheated and isotonic induced hemolytic study at 200 µg/ml.

Conclusion: Thus the present study results explains that EEZA is effective out of all the extracts and essential oil was potent. Thereby this plant can be used in the treatment of various inflammatory ailments.

Keywords: Syzygium aromaticum, in vitro, Diclofenac sodium, anti-inflammatory activity, EEZA

How to Cite

KASYAP, K., YESUDANAM, S., PRASANTH, M. L. L., & PRASANTH, C. R. S. (2021). AN In-vitro ANTI-INFLAMMATORY ACTIVITY OF HYDROALCOHOLIC EXTRACTS AND ESSENTIAL OIL OF SYZYGIUM AROMATICUM. Journal of International Research in Medical and Pharmaceutical Sciences, 16(3), 33–39. Retrieved from


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