Allelopathic Activity and Chemical Constituents of Extracts from Leaves and Stems of Kherchoun (Hertia cheirifolia)

Maroua Cherni

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie.

Sana Dallali

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie and Laboratoire des Ressources Sylvo Pastorales, Institut Sylvo-Pastoral de Tabarka, Université de Jendouba Tabarka, Tunisie.

Dhouha Dallali

Laboratory of Organic Chemistry (LR17ES/08) Natural Substances Section, University of Sfax, BP 1171, CP 3000, Sfax, Tunisie.

Rabiaa Mouhbi

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie.

Hanene Bouraoui

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie.

Saber Jdidi

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie.

Houcine Selmi

Laboratoire de Recherches en Systèmes de Production Agricole et de Développement Durable, Ecole, Supérieure d’Agriculture, Mograne, Zaghouan, Tunisie.

*Author to whom correspondence should be addressed.


Abstract

Allelopathy is a common biological phenomenon by which one organism produces biochemicals that influence the growth, survival, development, and reproduction of other organisms. The aim of this study was to investigate the phytochemical screening of the various extracts of Kherchoun (Hertia cheirifolia) and the evaluation of antioxidant capacities, by the DPPH test (2, 2-diphenyl-1-picrylhydrazyl), and of their allelopathic effects, vis-à-vis the two species: Vetch (Vicia sativa L.) and triticale (Triticosecale wittm L.). Biochemical analysis results showed that the highest total lipid content was observed at the leaf level (9%). The aqueous extracts of the leaves are rich in polyphenols, with a rate of 268.67mg EAG/g MS, compared to the stems (242.67mg EAG/g MS). The levels of flavonoids are higher in the leaves (38.67mg EAG/g MS) but lower in the stems (24.75mg EAG/g MS). On the other hand, the highest total tannin content recorded at leaf level is 608.33 mg EAG/g MS and the lowest is recorded in the stems (505 mg EAG/g MS). In contrast, the aqueous extract of the stems has the highest condensed tannin content (0.049 mg EAG/g MS), compared to that of the leaves (0.036 mg EAG/g MS). The two extract .Both aqueous extracts of Hertia cheirifolia are endowed with good antioxidant abilities. CI50 =139.43 2.99a μg/mL at leaf level and CI50 =111.38 22.2a μg/mL at stem level.Concerning the allelopathic study, the results obtained showed that the two extracts (Leaves and stems) of Hertia cheirifolia exerted different effects on the germination parameters of weed seeds of vetch (Vicia sativa L.) and triticale (Triticosecale wittm L.). Indeed, the two extracts exerted very important inhibitory effects, vis-à-vis the two seeds tested, on the germination and development of the seedlings (length of the root and length of the aerial part), in a significant and dose-dependent way.

Keywords: Hertia cheirifolia L., aqueous extract, secondary metabolites, antioxidant power, allelopathic activity


How to Cite

Cherni , M., Dallali , S., Dallali , D., Mouhbi , R., Bouraoui , H., Jdidi , S., & Selmi , H. (2023). Allelopathic Activity and Chemical Constituents of Extracts from Leaves and Stems of Kherchoun (Hertia cheirifolia). Journal of International Research in Medical and Pharmaceutical Sciences, 18(2), 1–12. https://doi.org/10.56557/jirmeps/2023/v18i28255

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References

Gupta Ankita and Mittal Chabbi. Effect of allelopathic leaf extract of some selected weed flora of ajmer district on seed germination of Triticum aestivum L. Science Research Reporter. 2012 ;2(3): 311-315.

Khalaj JaeHee Yoon Rhusheet Patel and Gemmy Hannsun. Therapeutic laquinimod treatment decreases inflammation initiates axon remyelination and improves motor deficit in a mouse model of multiple sclerosis brain and behavior. 2013; 3(6):664-682.

Farooq MKC, Flower K, Jabran A, Wahid Kadambot HM. Siddique. Crop yield and weed management in rainfed conservation agriculture. Soil and Tillage Research. 2011 ;117 :172-183

Moosavi A, Tavakkol Afshari R, Asadi A, Gharineh MH. Allelopathic Effects of Aqueous Extract of Leaf Stem and Root of Sorghum bicolor on Seed Germination and Seedling Growth of Vigna radiata L. Not Sci Biol. 2011;3(2):114-118.

Verdeguer M, Blazquez M.A, Boira H, 2009. Phytotoxic effects of Lantana camara Eucalyptus camaldulensis and Eriocephalus africanus essential oils in weeds of Mediterranean summer crops. Biochemical Systematics and Ecology 37: 362–369.

Marichali A, Hosni K, Dallali S, Ouerghemmi S, Bel Hadj Ltaief H, Benzarti S, Kerkeni A, Sebei H, Allelopathic effects of Carum carvi L. essential oil on germination and seedling growth of wheat maize flax and canary grass. Allelopathy Journal. 2014;34(1):81-94.

Khiari. Experimental investigation of Pistacia lentiscus biodiesel as a fuel for direct injection diesel engine. Energy. Convers. Manag. 2011;108:392-399.

Amri I, Hamrouni L, Hanana M. and Jamoussi B. Reviews on phytotoxic effects of essential oils and their individual components: news approach for weeds management. International Journal of Applied Biology and Pharmaceutical Technology. 2013;4:96-114.

Singh HP, Batish DR, Kohli RK. Allelopathic interactions and allelochemicals: New possibilities for sustainable weed management. Crit. Rev. Plant Sci. 2003;22:239–311.

Dayan FE, Cantrell CL, Duke SO. Natural products in crop protection. Bioorganic and Medicinal Chemistry. 2009;17:4022- 4034.

Wu H, Pratley J, Lemerle D, Haig T. Crop cultivars with allelopathic capability. Weed Res. 1999;39:171–180.

Jamil M, Cheema Z.A, Mushtaq M.N, Farooq M, Cheema MA. Alternative control of wild oat and canary grass in wheat fields by allelopathic plant water extracts. Agronomy for Sustainable Development. 2009;29:475-482.

Javaid, Anjum. Control of Parthenium hysterophorus L, by aqueous extracts of allelopathic grasses. Pakistan Journal of Botany. 2006 ;38:139–145.

Arslan M, Uremis I, Uludag A. Determining bio-herbicidal potential of rapeseed radish and turnip extracts on germination inhibition of cutleaf ground-cherry (Physalis angulata L.) seeds. Journal of Agronomy. 2005;4:134-137.

Mhamdi B, Aidi W. W, Chahed T, Ksouri R, Marzouk B. Phenolic compounds and antiradical scavenging activity changes during Borago officinalis stalk leaf development. Asian J. Chem. 2010; 22 :6397-6402.

Nandal DPS, Dhillon A. Allelopathic effects of poplar (Populus deltoides Bartr Ex Marsh): An assessment on the response of wheat varieties under laboratory and field conditions. 4the World Congress on Allelopathy; 2005.

Julkunen-Titto R. "Phenolic constituents in the leaves of northem wiliows methods forthe analysis of certain phenolics. Journal of Agricultural and Food Chemistry; 1985.

Neffati M, Sriti J, Hamdaoui G, Kchouk M.E, Marzouk B. Salinity impact on fruit yield essential oil composition and antioxidant activities of Coriandrum sativum fruit extracts. Food Chem. 2011;124:221–225.

Benhammou N, Atik Bekkara F, Kadifkova Panovsk T. Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. C. R. Chimie. 2009;12:1259–1266.

Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. 1959;37(8):911–917.

Dallali S, Lahmayer I, Mokni R, Marichali A, Ouerghemmi S, Bel Hadj Ltaief H, Sebei H. Phytotoxic effects of volatile oil from Verbena spp. on the germination and radicle growth of wheat maize linseed and canary grass and phenolic content of aerial parts. Allelopathy Journal. 2014;34(1):95-106.

Ghafarbi SP, Hassannejad S, Lotfi R. Allelopathic effects of wheat seed extracts on seed and seedling growth of eight selected weed species. International Journal of Agriculture and Crop Sciences (IJACS). 2012;4(19):1452-1457.

Inderjit S, Keating KI. Allelopathy principales procedures process and promises for biological control. Advances in Agronomy. 1999;67:141-231.

Jedidi et al. Comparative studies of phytochemical screening HPLC-PDAESIMS/MS-LC/HR-ESI-MS Analysis Antioxidant Capacity and in Vitro Fermentation of Officinal Sage (Salvia officinalis L.) Cultivated in Different Biotopes of Northwestern Tunisia; 2020.

Konstantinović. Allelopathic effect of weed species Amaranthus retroflexus L. on maize seed germination. Romanian Agricultural Research. 2014;4:116-121.

Kruse M, Strandberg M, Strandberg B. Ecological Effects of AllelopathicPlants: a Review. NERI Technical Report No. 315. National Environmental Research Institute Silkeborg Denmark. 2000;66.

Rahali N, Mehdi S, Younsi F, Boussaid M, Messaoud C. Antioxydant α-amylase and acetylcholinesterase inhibitory activities of Hertia cheirifolia L.essential oils: Influence of plant organs and seasonal variation Int. J. Food Prop. 2017 ;20:1637–165.

Ramachandra TV Kamaksi G Shutthi BV. Bioressource status in karnataka.Renewable and Sustainable energy Reviews. 2004 ;8:1-47.

Singleton VL Orthofer R Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteau reagent. Method. Enzymol. 1999;299:152–178.

Sun B, Ribes AM, Leandro MC, Belchior AP, Spranger MI. Stilbenes: Quantitative extraction from grape skins contribution of grape solids to wine and variation during wine maturation. Analytica Chimica Acta. 2011;563:382–390.

Trabelsi H, Cherif O. A, Sakouhi F, Villeneuve P, Renaud J, Barouh N, Boukchina S, Mayer P. Total lipid content fatty acids and 4-desmethylsterols accumulation in developing fruit of Pistacia lentiscus L. growing wild in Tunisia. Food Chemistry. 2012;131:2-6.

Uremis et al. Allelopathic effects of barley extracts on germination and seedlings growth of bread and durum wheats ; 2005.

Fabrizio Araniti Mariangela Marrelli Antonio Lupini Francesco Mercati Giancarlo Antonio Statti and Maria Rosa Abenavoli. Phytotoxic activity of Cachrys pungens Jan a mediterranean species: separation identification and quantification of potential allelochemicals. Acta Physiol Plant ; 2014. DOI 10.1007/s11738-013-1482-8

Scrivanti LR. Allelopathic potential of Bothriochloa laguroides var. laguroides (DC.) Herter (Poaceae: Andropogoneae). Flora. 2010;205:302-305.

Yu T, Lee YJ, Yang HM, Han S, Kim JH, Lee Y, Kim C, Han MH, Kim MY, Lee J, Cho JY. Inhibitory effect of Sanguisorba officinalis ethanol extracts on NO and PGE2 production is mediated by suppression of NF-Kb and AP-1 activation signaling cascade. J Ethnopharmacol. 2011;134:11-17.

Khanh TD, Chung MI, Xuan TD, Tawata S. The exploitation of crop allelopathy in sustainable agricultural production. J. Agron. Crop Sci. 2005;191:172-184.

Msaada K, Hosni K, BenTaarit M, Hammami M, Marzouk B. Effects of growing region and maturity stages on oil yield and fatty acid composition of coriander (Coriandrum sativum L.) fruit. Scientia Horticulturae. 2009;120:525–531.

Jemâa JMB. Essential oil as a source of bioactive constituents for the control of insect pests of economic importance in Tunisia. Med. Aromat. Plants. 2014;3(2): 158.

DOI: 10.4172/2167-0412.1000158.