Phytochemical Screening and Antimicrobial Evaluation of Ethanol and N-Hexane Extracts of Capsicum frutescens Fruit
Journal of International Research in Medical and Pharmaceutical Sciences, Volume 18, Issue 2,
Page 23-30
DOI:
10.56557/jirmeps/2023/v18i28399
Abstract
Medicinal plants have demonstrated strong promise as useful alternatives for the development of therapeutics with little or no side effects relative to synthetic drugs, as document by various studies. In this study, we evaluate the antimicrobial potentials of the methanol and n-hexane extracts of Capsicum frutescens fruit to establish its use in traditional medicine. The phytochemical and fatty acid profile is also presented. Phytochemical composition, fatty acid composition and antimicrobial activities of the extracts were performed following standard methods. Our results showed that while Tannins and phenols were not present in the n-hexane extract, alkaloids, flavonoids, saponins, glycosides and sterols were present. However, all phytochemicals evaluated were present in the ethanol extract. FTIR analysis of the extracts showed a strong absorption band at 3396 cm-1 for ethanol extract while the band is seen to diminish in the hexane extract. The band in the range 2978 – 2922 cm-1 was related to symmetric -CH3 stretching, 2855 - 2124 cm-1 due to C-H stretching, 1744 – 1640 cm-1 due to C=O stretching, 1461 – 1382 cm-1 due to -CH3CH bending of alkanes, 1159 – 1043 cm-1 due to =C-O-C- symmetric stretching and 879 – 723 cm-1 due to C-Cl stretching of alkyl halides as major spectral bands. Fatty acid profile showed the presence of several saturated and unsaturated fatty acids. The hexane and ethanol extracts respectively demonstrated significant antimicrobial potential against Escherichia coli (10.00±0.10 mm and 13.00±0.12 mm) and Staphylococcus aureus (11.00±0.21 mm and 13.00±0.03 mm) among other microbes compare to the control. These showed that the extract can be used as antimicrobial agent. Our data provides a preliminary explanation for the observed use of Capsicum frutescens fruits in traditional medicines for the treatment of bacterial infections.
- Antimicrobial
- phytochemistry
- ethanol extract
- fatty acids
How to Cite
References
Faur A, Ianovici N, Nechifor C. Air palynology research implications in allergic diseases. Annals of West University of Timişoara, ser. Biology. 2017;3-4:7-14.
Madueke AC, Ugwu DO, Nwanelo VO, Anajemba RC, Onoh PA, Anosike CA. Anti-Ulcerogenic effect of unripe plantain (Musa x Paradisiaca) pulp on indomethacin-induced ulcer in wistar albino rats. Tropical Journal of Natural Product Research. 2020;4(10):784-789.
Adeeyo AO, Odiyo J, Odelade K. Chemical profilingand antimicrobial properties of phyto-active extracts from Terminalia glaucescens stem against water microbial contaminants”: e Open Biotechnology Journal. 2018;12(1):232 - 238.
Ajayi O, Awala S, Ogunleye A, Okogbue F, Olaleye BF. “Antimicrobial screening and phytochemicalanalysis of Elaeis guineensis (ewe igiope) against salmonella strains,” British Journal of Pharmaceutical Research. 2016;10(3):1–9.
Egbuna C, Ifemeje JC, Kryeziu TL, Mukherjee M, Shah H. Introduction to Phytochemistry. American Journal of Essential Oils and Natural Products. 2018;5(2):223-226.
El-Ghani MMA. Traditional medicinal plants of Nigeria:an overview. Agriculture and Biology Journal of North America. 2016;7(5):220–247.
Esposito F, Carli I, Del Vecchio C. Sennoside A, derived from the traditional Chinese medicine plant rheum L., is a new dual HIV-1 inhibitor effective on HIV-1 replication. Phytomedicine, 2016; 23(12):1383–1391.
Ezike AC, Okonkwo CH, Akah PA, Okoye TC, Nworu CS. Landolphiaowariensis leaf extracts reduce parasitemia in Plasmodium berghei-infected mice. Pharmaceutical Biology. 2016;54(10): 2017–2025.
Kpadonou D, Kpoviessi S, Bero J, Agbani P, Gbaguidi F, Kpadonou-Kpoviessi B, Sinsin B, Frédérich M, Quetin-Leclercq J. Chemical composition, in vitro antioxidant and antiparasitic properties of the essential oils of three plants used in traditional medicine in Benin. Journal of Medicinal Plant Research. 2019;13(6):384–395.
Monika T, Karuna S, Renu K. Phytochemicals: extraction process, safety assessment, toxicological evaluations, and regulatory issues: Functional and Preservative Properties of Phytochemicals. 2020;4(2):221 -223.
Moussaoui F, Alaoui T. Evaluation of antibacterial activity and synergistic effect between antibiotic and the essential oils of some medicinal plants. Asian Pacific Journal of Tropical Biomedicine. 2016;6(1):32–37.
Ogbole OO, Akinleye TE, Segun PA, Faleye TC, Adeniji AJ. “In vitro antiviral activity of twenty-sevenmedicinal plant extracts from southwest Nigeria against three serotypes of echoviruses. Virology Journal. 2018;15(1):110.
Olasunkanmi OO, Akinpelu DA, Adeyemi PO, Ajayi OF, Omolofu-Aso J, Olorunmola FO. “Investigation into Antibacterial, Phytochemical and Antioxidant Properties of Vitellaria paradoxa (Gaertn.) Stem Bark Extracts”, Journal of Pharmaceutical Research International. 2017;20(5): 1-17.
Ozioko SC, Madueke AC, Anosike CA. Phytochemical composition and free radical scavenging activity of Cucurbita maxima fruit juice. Research Journal of Advanced Sciences. 2020;1(1):17- 26.
Okey EN, Madueke AC, Ossai EC, Anosike AC, Ezeanyika LUS. Anti-diarrhoeal properties of the ethanol extract of Terminalia glaucescens root on castor oil-induced diarrhea in wistar rats. Tropical Journal of Natural Product Research. 2020;4(8):446-450.
Madueke AC, Anosike CA. Anti-ulcerogenic effect of the chloroform extract of Chasmanthera dependens on indomethacin and aspirin-induced ulcer in rats. Journal of Nutritional Ecology and Food Research. 2017;4(2):131- 137.
Dutta AC. Botany for Degree Students. Sixth Edn. Oxford University Press. 2018;321-329.
Otunola GA, Oloyede OB, Oladiji AT, Afolayan JA. Comparative analysis of the chemical composition of three Spices- Allium sativum L., Zingiber officinale, Rosc. and Capsicum frutescens L. commonly consumed in Nigeria. Afr. J. Biotechnol. 2017;9(41):6927-6931.
Oni MO. Evaluation of seed and fruit Powders of Capsicum annum and Capsicum frutescens for control of Callosobruchus maculates (F.). Int. J. Biol. 2016;3(2):185-188.
Tyowua AT, Agbidye IG. Antimicrobial activity of leaf extracts of Phragmantheraincana on Salmonella typhi, Escherichia coli and Staphylococcus aureu. Nigerian Journal of Pure and Applied Sciences. 2016;3(1): 49-52.
Sharma AP, Tripathi BD. Assessment of atmospheric PAHs profile through Calotropis gigantean R.Br. leaves in the vicinity of an Indian coal-fired power plant. Environmental Monitoring Assessment. 2019;149(12):477 –482.
Sureshkumar P, Chezhian A, Senthil RP, Sathiyapriya J. Computational selections of terpenes present in the plant Calotropis gigantea as mosquito larvicide’s by blocking the sterol carrying protein, Bangladesh Journal of Pharmacolog. 2018;7(2):1-5.
Shanmugam A, Subhapradha N, Suman S, Ramasamy P, Saravanan R, Shanmugam V, Srinivasan A. Characterization of biopolymer “chitosan” from the shell of donacid clam Donax scortum (Linnaeus, 1758) and its antioxidant activity. International Journal of Pharmaceutical and Pharmacological Sciences. 2019;4(2) :460−465.
Nwokem CO, Agbaji EB, Kagbu JA, Ekanem EJ. Determination of Capsaicin content and pungency level of five different peppers grown in Nigeria. New York Sci. J. 2018;3(9):17-21.
Murti PB, Seshadri TR. Chemical composition of Calotropis gigantea: Part V. Further Examination of the Latex and Root Bark, Proc. Indian Academic Journal of Sciences. 2021;21(3):143–147.
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