Generation and Evaluation of Genomic Markers in Grapes (Vitis vinifera L.)
Pooja B. Patole *
VDCOAB, Latur, India.
Rutuja S. Deshamukh
VDCOAB, Latur, India.
R. N. Dhawale
Departement of Animal Biotechology, VDCOAB, Latur, India.
*Author to whom correspondence should be addressed.
Abstract
The present study focused on the identification and validation of simple sequence repeat (SSR) markers associated with important berry traits in grapevine (Vitis vinifera L.). Young leaf samples from four grape genotypes and ten hybrids were used for DNA extraction and molecular analysis. High-quality genomic DNA was successfully isolated and quantified, confirming its suitability for PCR amplification. Microsatellite regions were identified from candidate gene sequences using the MISA tool, and primers were designed with Primer3Plus. Out of five designed SSR primers, four exhibited polymorphism among grape genotypes, while two demonstrated polymorphism in hybrid populations. These validated SSR markers serve as potential tools for genetic diversity analysis and marker-assisted selection in grape breeding programs aimed at improving berry quality traits.
Keywords: Vitis vinifera, SSR markers, polymorphism, berry traits