Journal of Biology and Nature

The present study was conducted to evaluate and compare haemato-biochemical parameters in dogs with long bone diaphyseal fractures stabilised using locking compression plate (LCP) alone (Group I, n=6) and LCP augmented with composite bone graft containing bioactive glass and platelet-rich plasma (PRP) (Group II, n=6). The composite graft was used to enhance bone healing by providing osteoconductive support (bioactive glass) and growth factors (PRP). The study also aimed to assess changes in these parameters at different postoperative time intervals and to determine their usefulness as indicators of fracture healing. It was hypothesized that the addition of PRP and bioactive glass would improve fracture healing compared to LCP fixation alone. Haematological parameters including haemoglobin (Hb), packed cell volume (PCV), total leucocyte count (TLC) and differential leucocyte count, and biochemical parameters including serum calcium, phosphorus and alkaline phosphatase (ALP) were evaluated preoperatively (0^th day) and postoperatively on 15^th, 30^th, 45^th and 60^th days. The data collected were statistically analyzed using SPSS version 20. Analysis of variance (ANOVA), followed by Tukey's HSD post hoc test, was used to compare variations in hematological and biochemical parameters at different preoperative and postoperative time intervals. Among the parameters studied, haemoglobin, serum calcium and alkaline phosphatase showed significant difference between the groups, with higher values observed in Group II, indicating enhanced bone healing activity. The findings suggested that haemato-biochemical parameters were useful indicators of fracture healing and that augmentation of LCP with composite bone graft improved the healing response compared to LCP alone.