OPTIMIZATION OF CULTURE CONDITIONS FOR AMYLASE PRODUCTION FROM LOCALLY ISOLATED Aspergillus sp.
ARUN KUMAR SHARMA
Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
VINAY SHARMA *
Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
ARINDAM KUILA
Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
JYOTI SAXENA
Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, India
*Author to whom correspondence should be addressed.
Abstract
In the present study, two locally isolated fungal strains (Aspergillus sp. 1 and Aspergillus sp. 2) were used for amylase production under submerged fermentation. Before optimization, Aspergillus sp. 1 and 2 demonstrated maximum amylase production of 82 ± 0.79 IU/mL and 110 ± 0.32 IU/mL, respectively. One variable at a time approach was used for optimization of culture conditions such as carbon source, nitrogen source, pH and incubation time. Optimized parameters for increased amylase production from Aspergillus sp. 1 was found to at pH 10.0 after 4 days of incubation using fructose as carbon source and ammonium sulfate as nitrogen source. Similarly, Aspergillus sp. 2 demonstrated increased amylase production at pH 10.0 of production medium after 4 days of incubation using fructose as carbon source and peptone as nitrogen source. After single parameter optimization amylase activity from Aspergillus sp. 1 and Aspergillus sp. 2 was found to be 161.23 ± 1.28 IU/mL and 166.73 ± 1.12 IU/mL, respectively. Further, response surface methodology (RSM) based central composite design (CCD) was used for optimization of amylase production from Aspergillus sp. 2. Maximum amylase production (172.36 IU/mL) was found using 1% sucrose concentration, 1.05% peptone concentration at pH 11 after 5 days of incubation time.
Keywords: Aspergillus sp, amylase, submerged fermentation, culture condition optimization, response surface methodology