SCAVENGING ANTIOXIDATIVE BIOACTIVITIES OF Solanecio biafrae (Wòròwó) IN RABBITS INDUCED WITH ALCOHOL TOXICITY
MATHEW FOLARANMI OLANIYAN *
Department of Medical Science, Achievers University, Owo, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Background: Solanecio biafrae (Èfó Wòròwó) is an important vegetable plant commonly grown in cocoa plantation in South-West geopolitical zone of Nigeria majorly in Ondo and Ekiti-States which has some traditional treatment claims such as cough-cure, for heart-troubles, to stop bleeding from cuts, rheumatic pain, prurigenic, allergies and localized oedemas.
Aim and Objective: This study was designed to determine Scavenging antioxidative bioactivities of Solanecio biafrae (Wòròwó).
Materials and Methods: Twenty five rabbits classified into 5 groups of five rabbits each weighed 1.11 ±0.2 Kg (Variance: 0.04). The groups were labeled as A, B, C, D(D1, D2), E (E1, E2); group A was fed on normal meal, group B and C were given 500 mg/Kg of ethanolic and aqueous extract of Solanecio biafrae (Wòròwó) respectively for 7 days; Group D1 and E1 were induced for oxidative stress by administering 15 ml of 40% ethanol by intubation for 3 weeks; D2 and E2 after oxidative stress inducement were given 500 mg/Kg of ethanolic and aqueous extract of Solanecio biafrae (Wòròwó) respectively for 7 days. Oxidative stress and antioxidative bioactivities of the extract were determined biochemically by spectrophotometry in the experimental rabbits and control using plasma concentration of Malondialdehyde (MDA); plasma activities of catalase (CAT), superoxide dismutase. (SOD) and Glutathione peroxidase (GPx).
Results: The result obtained showed a significant increase in MDA and a significant decrease in CAT, SOD and GPx when the rabbits were given administering 15 ml of 40% ethanol by intubation for 3 weeks with p<0.05. There was also a significant decrease in MDA and a significant increase in CAT, SOD and GPx when the rabbits were given 15 ml of 40% ethanol for 3 weeks (p<0.05). Administration of ethanolic and aqueous extract of Solanecio biafrae (Wòròwó) in rabbits showed a significant decrease in MDA than the normal control (p<0.05). There was a significant increase in plasma MDA when the rabbits were given ethanolic extract of Solanecio biafrae (Wòròwó) with p<0.05.
Conclusion and Recommendation: Findings of this work on the bioactivities of ethanolic and aqueous extract of Solanecio biafrae (Wòròwó) could therefore be associated with possible scavenging anti oxidative properties. Solanecio biafrae (Wòròwó) aqueous and ethanol extract could therefore be used as a dietary supplement to reduce oxidative stress.
Keywords: Solanecio biafrae extract, CAT, SOD, MDA, GPx, scavenging, antioxidative stress, antioxidant, bioactivities