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We investigated the effect of humic acid and new Zn nano fertilizer derived from some physiological traits and yield of Summer savory plant under greenhouse conditions. The treatments consisted of four levels of each of humic acid (0.0, 0.5, 1.0 and 1.5 mg/L) and nano Zn chelated fertilizer (0.0, 50, 100 and 200 mg/L). The application of humic acid and nano Zn chelated fertilizer alone and together increased amounts of studied characteristics. The highest amounts of these traits were obtained from the simultaneous utilization of both types of fertilizers. The combined use of these fertilizers improved the length of root and increased to access to more volume of soil. The enhanced root growth enabled the plant to uptake more nutrients resulting in improved growth and yield. A significant increase of leaf width (4 cm), number of branches (20) root length (12.7 cm), iron concentration (21.25 mg kg-1), zinc (0.95 mg kg-1), nitrogen (1.21 mg/g/fw) and potassium concentration (53.68 ppm), essential oil percent (2.58%) and yield (47.81 mg/pot) of savory were recorded in the application) H4=1.5 mg/L humic acid and N4= 200 mg/L nano Zn chelated) compared to the control (N1=0 nano Zn chelated and H1=0 humic acid). The study suggests that combined use of humic acid and the nano Zn chelated fertilizers can have greater impact on increasing the yield and quality of products such as savory.

Essential oil, foliar application, humic acid, growth and yield, nano zinc, root length, savory

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VAFA, Z. N., SOHRABI, Y., & SAMIR, Z. (2020). HUMIC ACID AND NANO ZN CHELATED FERTILIZER REGULATES NUTRIENT UPTAKE AND GROWTH AND PRODUCTION OF Summer savory. Journal of Biology and Nature, 11(4), 5-15. Retrieved from
Original Research Article


The effect of foliar application of fulvic acid on water use, nutrient uptake and yield in wheat. Australian J Agri Res. 1986;37(4):343-350.

Seifi Nadergoli MS, Yarnia M, Khoei FR. Effect of zinc and manganese and their application method on yield and yield components of common bean (Phaseolus vulgaris L. CV. Khomein). Middle East J Scienti Res. 2011;8(5):859-865.

Derakhshani Z, Hassani A, Sadaghiani MHR, Hassanpouraghdam MB, Khalifani BH, Dalkani M. Effect of zinc application on growth and some biochemical characteristics of costmary (Chrysanthemum balsamita L.). Commun Soil Sci and Plant Anal. 2011; 42(20):2493-2503.

Fedorenko VF, Buklagin DS, Golubev IG, Nemenushchaya LA. Review of Russian nanoagents for crops treatment. Nanotechnol in Russia. 2015;10(3-4):318-324.

Scaglia B, Pognani M, Adani F. Evaluation of hormone-like activity of the dissolved organic matter fraction (DOM) of compost and digestate. Sci Tot Env. 2015;514:314-321.

Chen Y, De Nobili M, Aviad T. Stimulatory effects of humic substances on plant growth. Soil Org Matt In Sust Agric. 2004;103-129.

Rezvanpanah S, Rezaei K, Golmakani MT, Razavi SH. Antibacterial properties and chemical characterization of the essential oils from summer savory extracted by microwave-assisted hydrodistillation. Brazilian Journal of Microbiology. 2011;42(4):1453-1462.

Singh NB, Amist N, Yadav K, Singh D, Pandey JK, Singh SC. Zinc oxide nanoparticles as fertilizer for the germination, growth and metabolism of vegetable crops. J Nanoengg and Nanomanuf. 2013;3(4):353-364.

Fong SS, Seng L, Mat HB. Reuse of nitric acid in the oxidative pretreatment step for preparation of humic acids from low rank coal of Mukah, Sarawak. J Brazilian Chem Soc. 2007;18(1):41-46.

Sanchez-Conde MP, Ortega CB. Effect of humic acid on the development and the mineral nutrition of the pepper plant. Cont de la Fert de las Plant Culti. 1968;2:745-755.

Stephan WK, Charles WJ. Experimentation with Arkansas lignite to identify organic soil supplements suitable to regional agricultural needs. Proposal Arkansas Technology University. Russellville. 1994;AR72801(501): 968-0202.

Atiyeh RM, Lee S, Edwards CA, Arancon NQ, Metzger JD. The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Biores Technol. 2002; 84(1):7-14.‏

Pettit RE. Organic matter, humus, humate, humic acid, fulvic acid and humin: Their importance in soil fertility and plant health. CTI Research. 2004;1-17.

Fageria NK, Stone LF. Micronutrient deficiency problems in South America. In Micronutrient deficiencies in global crop production. Springer, Dordrecht. 2008;245-266.

Nyawade SO, Karanja NN, Gachene CK, Gitari HI, Schulte-Geldermann E, Parker ML. Short-term dynamics of soil organic matter fractions and microbial activity in smallholder potato-legume intercropping systems. Appl Soil Ecol. 2019;142:123-135.

Adhikari T, Kundu S, Biswas AK, Tarafdar JC, Subba Rao A. Characterization of zinc oxide nano particles and their effect on growth of maize (Zea mays L.) plant. J Plant Nutr. 2015;38(10):1505-1515.‏

Malakuti MJ, Lotfollahi MA. Role of Zn in qualitative and quantitative increase of agricultural crops and improving society health. Amuzesh Keshavarzi Press; 1999.

Ziaeian AA, Malakuti MJ. Studying the greenhouse effects of consuming Fe, Mn, Zn, and Cu on wheat production in intensive limewater soils of Fars Province. Wheat Moderate Nutrition. The collection of articles. Amuzesh Keshavarzi Press. 2000;544.

Gitari HI, Karanja NN, Gachene CK, Kamau S, Sharma K, Schulte-Geldermann E. Nitrogen and phosphorous uptake by potato (Solanum tuberosum L.) and their use efficiency under potato-legume intercropping systems. Field Cro Res. 2018;222:78-84.

Ayas H, Gulser F. The effects of sulfur and humic acid on yield components and macronutrient contents of spinach (Spinacia oleracea Var. Spinoza). J. Biol Sci. 2005;5: 801-804.

Çimrin KM, Türkmen Ö, Turan M, Tuncer B. Phosphorus and humic acid application alleviate salinity stress of pepper seedling. African J Biotechnol. 2010;9(36): 5845-5851.

Rafique R, Zahra Z, Virk N, Shahid M, Pinelli E, Park TJ, Arshad M. Dose-dependent physiological responses of Triticum aestivum L. to soil applied TiO2 nanoparticles: Alterations in chlorophyll content, H2O2 production, and genotoxicity. Agri, Ecosyst & Env. 2018;255:95-101.

Selladurai R, Purakayastha TJ. Effect of humic acid multinutrient fertilizers on yield and nutrient use efficiency of potato. J Plant Nutr. 2016;39(7):949-956.

Mylonas VA, McCants CB. Effects of humic and fulvic acids on growth of tobacco. Plant and soil. J Plant Nutr. 1980;54(3):485-490.

Mylonas VA, McCants CB. Effects of humic and fulvic acids on growth of tobacco. Plant and soil. J Plant Nutr. 1980;54(3):485-490.

International Organization for Standardization (Genève). Soil quality: Determination of effective cation exchange capacity and base saturation level using barium chloride solution. ISO; 1994a.

Sarrwy SMA, Mohamed EA, Hassan HSA. Effect of foliar sprays with potassium nitrate and mono-potassium phosphate on leaf mineral contents, fruit set, yield and fruit quality of picual olive trees grown under sandy soil conditions. American-Eurasian J Agric and Env Sci. 2010;8(4):420-430.

Marschner H. Mineral nutrition of higher plants. Academic Pres. Inc., San Diago; 1995.

International Organization for Standardization (Genève). Soil quality: Determination of effective cation exchange capacity and base saturation level using barium chloride solution. ISO; 1994b.

Hofman G, Van Cleemput O. Soil and plant nitrogen. International Fertilizer Industry Association Paris, France; 2004.

Nurzyńska-Wierdak R. Does mineral fertile-zation modify essential oil content and chemical composition in medicinal plants. Acta Scientiarum Polonorum-Hortorum. 2013;12:3-16.

Yassen AEAA, EAA, AEN, Shedeed S. Response of wheat to foliar spray with urea and micronutrients. J American Sci. 2010;6(9):14-22.

Erdal I, Kepenek K, Kizilgöz İ. Effect of foliar iron applications at different growth stages on iron and some nutrient concentrations in strawberry cultivars. Turkish J. Agric and For. 2004;28(6):421-427.

Said-Al Ahl HAH, Mahmoud AA. Effect of zinc and/or iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum L.) under salt stress. Ozean J Appl Sci. 2010;3(1):97-111.

Said-Al Ahl HAH, Omer EA. Effect of spraying with zinc and/or iron on growth and chemical composition of coriander (Coriandrum sativum L.) harvested at three stages of development. J Med Food Plants. 2009;1(2):30-46.

Akhtar N, Sarker MAM, Akhter H, Nada MK. Effect of planting time and micronutrient as zinc chloride on the growth, yield and oil content of Mentha piperita. Bangladesh J Sci Ind Res. 2009;44(1):125-130.‏

Akhtar W, Sharif M, Shah H. Competitiveness of Pakistani fruits in the world market.‏ Lahore Sch Eco J. 2009;14:125-133.

Nasiri Y, Zehtab-Salmasi S, Nasrullahzadeh S, Najafi N, Ghassemi-Golezani K. Effects of foliar application of micronutrients (Fe and Zn) on flower yield and essential oil of chamomile (Matricaria chamomilla L.). J of Med Plants Res. 2010;4(17):1733-1737.

Hassanpouraghdam MB, Gohari GR, Tabatabaei SJ, Dadpour MR, Shirdel M. NaCl salinity and Zn foliar application influence essential oil composition of basil (Ocimum basilicum L.). Acta Agri Slovenica. 2011; 97(2):93-98.

International Organization for Standardization (Genève). Soil quality: Determination of total nitrogen: Modified Kjeldahl Method. ISO; 1995.

International Organization for Standardization (Ginebra). ISO 14235: Soil Quality: Determination of Organic Carbon by Sulfochromic Oxidation. ISO; 1998.

Zare DS, Asrar Z, Mehrabani M. Biochemical changes in terpenoid compounds of Mentha spicata essential oils in response to excess zinc supply. Iranian J Plant Biol. 2010;2:1-10.

Madison WI. Evaluation of iron catalysts for the Fenton-like remediation of diesel-contaminated soils. USA: American Society of Agronomy. 1996;539-579.

Salehin F, Rahman S. Effects of zinc and nitrogen fertilizer and their application method on yield and yield components of Phaseolus vulgaris L; 2012.

Abu-Muriefah SS. Effect of chitosan on common bean (Phaseolus vulgaris L.) plants grown under water stress conditions. Intl Res J Agric Sci and Soil Sci. 2013;3(6):192-199.‏

Alizadeh A, Khoshkhui M, Javidnia K, Firuzi O, Tafazoli E, Khalighi A. Effects of fertilizer on yield, essential oil composition, total phenolic content and antioxidant activity in Satureja hortensis L. (Lamiaceae) cultivated in Iran. J Med Plant Res. 2010;4(1):33-40.