EXPRESSION OF ANTIGEN THOMSEN–FRIEDENREICH RECOGNIZED BY Amaranthus leucocarpus LECTIN IN MCF CELLS STIMULATED WITH LIPOPOLYSACCHARIDE
LUIS MIGUEL GARCÍA CRUZ
Laboratorio de Genómica, Proteómica y Glicobiología del Cáncer, Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina, Universidad Autónoma “Benito Juárez” de Oaxaca, Oaxaca Oax, México.
IVÁN ANTONIO GARCÍA MONTALVO
Instituto Tecnológico de Oaxaca, Tecnológico Nacional de México, Esquina Calzada Tecnológico, Oaxaca de Juárez, Oaxaca, México.
BERENICE FERNÁNDEZ ROJAS
Laboratorio de Genómica, Proteómica y Glicobiología del Cáncer, Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina, Universidad Autónoma “Benito Juárez” de Oaxaca, Oaxaca Oax, México.
ITANDEHUI BELEM GALLEGOS VELASCO
Laboratorio de Genómica, Proteómica y Glicobiología del Cáncer, Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina, Universidad Autónoma “Benito Juárez” de Oaxaca, Oaxaca Oax, México.
PEDRO ANTONIO HERNÁNDEZ CRUZ *
Laboratorio de Genómica, Proteómica y Glicobiología del Cáncer, Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina, Universidad Autónoma “Benito Juárez” de Oaxaca, Oaxaca Oax, México.
*Author to whom correspondence should be addressed.
Abstract
Altered glycosylation is a characteristic of cancer cells, where the overexpression of truncated antigens such as the Thomsen–Friedenreich (TF) antigen (Galβ1, 3GalNAcα1, O-Ser / Thr) The TF antigen is specifically recognized by the Amaranthus leucocarpus (ALL). The MCF-7 breast cancer cell line maintains characteristics similar to differentiated mammary epithelial cells. It has been linked to stimulation of breast cancer cells with lipopolysaccharide, with a higher risk of metastasis in this type of cancer. In this work the expression of TF antigen and moesin in MCF-7 stimulated with LPS at concentration of 20 ng / ml cells was determined, by flow cytometry and fluorescence microscopy. MCF-7 cells express 45% of TF antigen without stimulus with LPS, the expression of the TF antigen decreases with the time of LPS stimulation. Moesin expression increases with LPS stimulation time. A double-positive MCF-7 population for moesin and a TF-antigen of 6.34% were identified by cytometry at 6 hours of LPS stimulation, compared to cells with no stimulus, whose double positivity was 2.86%. Our results suggests that moesin could be Amaranthus leucocarpus receptor in MCF-7 cells.
Keywords: Lipopolysaccharide, lectin, MCF-7, O-glycosylation