Effect of Gotu Kola (Centella asiatica) on Haematology, Immune Response and Disease Resistance of Common Carp (Cyprinus carpio)
Monisha, Y. H
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
Ganapathi Naik, M.
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
T. J. Ramesha
ICAR-KVK, KVAFSU, Kankanady Post, Mangaluru, India.
T. Suresh
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
Annappaswamy, T. S.
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
Ravindragouda Patil *
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
Rakesh, K
College of Fisheries, KVAFSU, Kankanady Post, Mangaluru, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Dietary influence of Gotu kola (Centella asiatica) on haematology, immune response and disease resistance was assessed in common carp (Cyprinus carpio) at the Research and Instructional Fish Farm, College of Fisheries, KVAFSU, Mangaluru, Karnataka, India and the experiment was carried out for a duration of 90 days. Four test diets were formulated (T1, T2, T3, and T0) each containing 30 % protein and gotu kola powder was added at varying levels: 5 g/kg in T1, 10 g/kg in T2, and 20 g/kg in T3 with each treatment replicated three times. The control diet(T0) contained no Gotu Kola powder. The superior activity of lysozyme and super oxide anion production were noted in T3 diet compared to other treatments and control group. After challenging against Aeromonas hydrophila, T3 group demonstrated 100 % survival rate. Administration of Gotu Kola diet made a greater impact on haematological parameters of common carp in all the treatments especially T3 group when compared with control. Hence, from the present study it is evident that incorporation of 20 g/kg Gotu kola in the diet can significantly improve haematological indices, immune response and disease resistance of common carp.
Keywords: Centella asiatica, Cyprinus carpio, haematology, NBT, lysozyme, disease resistance, Aeromonas hydrophila