Methyl Jasmonite Induced Elicitation of the Biosynthesis of Bonducellin – A Homo Isoflavanoid in the Cell Suspension Culture of Leaf Derived Callus of Caesalpinia bonducella (L) Roxb
Manju Rakesh
*
Modern College of Arts, Science and Commerce, Shivaji Nagar, Pune, Maharashtra, India.
Neeta M Patil
Modern College of Arts, Science and Commerce, Shivaji Nagar, Pune, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
Caesalpinia bonducella (L) Roxb., a significant ethno medicinal plant is a repository of bioactive compounds. Bonducellin is an important secondary metabolite found in Caesalpinia bonducella (L) Roxb., which possess antioxidant, anti-inflammatory, anticancer, antispasmodic, antihelminthic, anti-sterility and also used in the treatment of Polycystic Ovarian Syndrome (PCOS). The over exploitation of the plant is drastically reducing the natural population. Hence, the conservation strategies should be implemented by providing an in vitro alternative for the production of pharmacologically important compounds like bonducellin to retain the present status of the plant. The main objective of the present study is to enhance the in vitro production of bonducellin using abiotic elicitor methyl jasmonite. The leaf derived callus obtained in the Murashige and Skoog (MS) medium treated with 1 ml methyl jasmonite for 10 days produced 3.42 ± 0.02 µ g/g DW bonducellin. Thus, the abiotic elicitor methyl jasmonite can be utilized for the large-scale industrial production of bonducellin.
Keywords: Bonducellin, Methyl jasmonite, LCMS, HPLC, Caesalpinia bonducella (L) Roxb