ANTI-DIABETIC ACTIVITY OF Calotropis gigantea IN HUMAN WHOLE BLOOD
AMIT GUPTA *
Department of Immunology, Vidya Pratishthan’s School of Biotechnology, Baramati, District Pune, Maharashtra, India
SUSHAMA R. CHAPHALKAR
Department of Immunology, Vidya Pratishthan’s School of Biotechnology, Baramati, District Pune, Maharashtra, India
*Author to whom correspondence should be addressed.
Abstract
The aim of our present study is to evaluate the anti-diabetic activity of proteases extracted from specific protein antigen, bovine serum albumin (BSA) using Calotropis gigantea, flowers dissolved in phosphate buffered saline (PBS, acidic pH 5). In this study, treatment of acidic proteases of different concentration on diabetic human whole blood samples in order to cross-examine its blood counts (lymphocytes, monocytes and granulocytes count) and also analyzed forward scatter (shape and size) and side scatter (granularity of the cell) using flow cytometry. The results showed that acidic protease enhanced the granulocytes count which is generally useful aspect in order to prevent the cardiovascular infections and also retained the monocytes count in normal range and also showed that there is enhancement in the count of forward and side scatter in case of diabetic human blood samples. Overall, the results showed that Calotropis gigantea showed anti-diabetic activity.
Keywords: Calotropis gigantea, proteases, anti-diabetic