ANTIBIOGRAM OF MICROBES ASSOCIATED WITH ENTERIC INFECTION TARGETING INCIDENCES OF MULTIDRUG RESISTANCE
SHIVANGI DIXIT
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali, Tonk- 304022, Rajasthan, India.
SARIKA GUPTA *
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali, Tonk- 304022, Rajasthan, India.
*Author to whom correspondence should be addressed.
Abstract
Background and Objective: The Enterobacteriaceae family includes several genera that are biochemically and genetically related to each other and that cause primary gastrointestinal tract infections. Members of this family are Enterobacter spp., Escherichia spp., Klebsiella spp., and Pseudomonas spp., etc. major causes of opportunistic infection (including Diarrhea, Dysentery, Shigellosis, and Salmonellosis). The multidrug-resistant organisms (MDROs) are a major public health problem, on a global level relate to significant mortality and morbidity. Enterobacteriaceae reduced resistant to penicillin’s, broad-spectrum ESBLs production cephalosporin and monobactams are known as the main cause of both community and nosocomial-acquired infections.
Methods: The samples were collected as raw unprocessed and processed food. Preliminary all the samples were subjected to microbiological analysis for the isolation and identification of microbial species in the test samples. It is followed by the assessment of the resistance index of different antibiotics.
Results: 12 food samples were collected and analyzed microbiologically. A total of 32 bacterial isolates were recovered from different samples. The present data revealed the occurrence of 65.2% Gram-Positive bacteria and 34.7% Gram-Negative bacteria among the screened microbial isolates. The potential Enterobacteriaceae were found to be 99.9% among gram-negative isolates as Klebsiella pneumoniae, Enterobacter aerogenes, E. coli, Pseudomonas aeruginosa. The bacterial isolates reported maximum resistance to Cefazolin (82.35%) followed by maximum resistance to Ampicillin (47.05%), Lemefloxacin and Cefixime (41.17%), Cefuroxime and Nitrofurantoin (29.41%), Cotrimoxzole and Imipenem (23.5%), Fosfomycin (17.6%), Amoxyclav and Norfloxacin (11.76%), Amikacin, Gentamicin, Cefpotoxime, Piperacillin, Ticarcillin, and Meropenem (5.88%).
Conclusion: The present study investigates and assesses incidences of microbes associated with enteric infection from the raw and processed food and to characterize them on the basis of antibiogram, thereby analysis drug resistance among the population of bacteria.
Keywords: Enterobacteriaceae, food borne disease, food borne illness, multidrug resistant