Main Article Content
Stem disc explant of Aloe was cultured on MS basal medium containing different auxins, cytokinins as sole as well as in combinations. Culture medium MS2D (MS+2.0 mg.l-1 2,4-D) initiated callus in higher proportion. Proliferation medium MS3B (MS+3.0 mg.l-1 2,4-D) demonstrated higher shoot proliferation efficiency (90.41%) as well as number of shoot (s) / responding explant (9.67), while nutrient medium MS3B.5N (MS+3.0 mg.l-1 NAA+ 0.5 mg.l-1NAA) produced shoot of higher length (7.04 cm). Higher in vitro rooting response viz: root proliferating efficiency (92.88%), number of root (s) (9.75 ) and mean root length (9.10 cm) was shown by rooting medium MS2IB (MS+ 2.0 mg.l-1 IBA). Regenerated plantlets were established successfully in the field after hardening. Phenotypically normal plants were regenerated.