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To search out the responsive culture medium combination with higher regeneration potential, stem disc of Polianthes tuberosa was cultured on MS basal medium fortified with different auxins and cytokinins in varying concentrations and combinations. Culture medium Wh4D (Wh + 4.0 mg.l-12,4-D) proved well for callus initiation. Inoculation medium WhTd.5N (WH + 1.0 mg.l-1TDZ+ 0.5 mg.l-1NAA) exhibited higher shoot proliferating efficiency, while number of shoot (s) per explant in higher umber (s) was attained on culture medium Wh2Td.5N (Wh + 2.0 mg.l-1TDZ + 0.5 mg.l-1NAA). Nutrient medium WH.5B.5N (Wh + 0.5 mg.l-1 BA+ 0.5 mg.l-1 NAA) produced shootlet of higher length. Higher in vitro rooting response (root proliferating efficiency, number of root (s) and root of higher length) was displayed by rooting medium WhI (Wh + 1.0 mg.l-1IBA). In order to regiment low-cost effective mass in vitro propagation protocol it was evident that there is no influential difference in response of cultured stem discs on different culture media, supplemented either with purified or commercial grade sucrose as well as with purified or commercial grade bacto agar. Thus the cost of in vitro mass propagation can be reduced significantly by supplementing commercial grade sucrose and bacto agar instead of purified sucrose and agar respectively in culture medium.