EFFECTS OF In vitro PROPAGATION ON ONTOGENY OF Rosa canina L. MICROPROPAGATED PLANTS AS A PROMISING ROOTSTOCK FOR ORNAMENTAL ROSES
ELENA V. AMBROS *
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
OLGA YR. VASILYEVA
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
TATYANA IV. NOVIKOVA
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
*Author to whom correspondence should be addressed.
Abstract
A protocol for in vitro propagation through axillary buds culture for a promising winter hardy form of Rosa canina used as a rootstock for ornamental roses was developed.
Axillary meristems isolated in September-October were successfully established for regeneration of adventitious shoots in hormone-free modified liquid Murashige and Skoog (MS) medium with 100 mg l-1 of glutathione and 3% (w/v) glucose in darkness for 2-3 days followed by subculture on induction MS medium supplemented with 2.0 mg l-1 benzyladenine (BA) and 1.0 mg l-1 indole-3-acetic acid (IAA). The highest multiplication rate (7.5 shoots per explant) was archieved on MS medium supplemented with 1.0 mg l-1 BA. Rooting was induced on half-strength MS medium supplemented with 1.0 mg l-1 IAA. The regenerated plants were acclimatized in containers with sterile sand followed by transferring into pots containing a peat with perlite, mold, sand and coconut substrate (1:1:0.5:0.5) and finally were planted in the field. The use of biotechnological techniques allowed obtaining uniformed clonal material of rootstocks for ornamental roses with the best parameters of inoculation zone, compared with the plants propagated by green cutting.
Keywords: Rosa canina, axillary bud, adventitious shooting, in vivo growth and development, ontogeny