SEARCH FOR THE METHODS OF THE OPTIMAL REPRODUCTION OF Begonia variegata IN CONNECTION WITH ITS ANTIMICROBIAL PROPERTIES
Alexandra YU. Nabieva *
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
Tatyana D. Fershalova
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
Natalya V. TsybulYa
Laboratory of Biotechnology, Central Siberian Botanical Garden of the Siberian Branch, RAS, Zolotodolinskaya St., 101, 630090, Novosibirsk, Russia
*Author to whom correspondence should be addressed.
Abstract
Comparative analysis of seasonal changes in the antimicrobial activity of volatile components emitted by the leaves of Begonia variegata Y. M. Shui & W. H. Chen was carried out against potentially pathogenic species Staphylococcus epidermidis, Escherichia coli and Candida albicans on the basis of colony growth inhibition by a piece of leaf, followed by score-based estimation of the activity. This study presents the influence of exogenous cytokinins (TDZ, BA) and auxin IBA, added into the modified nutritive media MS and N6, in modeling of in vitro micropropagation of B. variegata starting from such explants as immature flower buds. The conventional propagation method involving the studies of the rhizogenesis response of begonia leaf cuttings in 4 different substrates was evaluated in comparison with biotechnological approaches to B. variegata multiplication.
Keywords: Begonia, antimicrobial activity, volatile emissions, micropropagation, plant growth regulators, flower buds, rhizogenesis, leaf cuttings