BIOSYNTHESIS OF PHYTOECDYSTEROIDS IN THE Hairy root CULTURE OF Silene linicola C. C. Gmelin
ANNA ALEKSEEVNA ERST *
Central Siberian Botanical Garden of SB RAS, Zolotodolinskaya St., 101, Novosibirsk, 630090, Russia
TATIANA VITALIEVNA ZHELEZNICHENKO
Central Siberian Botanical Garden of SB RAS, Zolotodolinskaya St., 101, Novosibirsk, 630090, Russia
ANNA ALEXANDROVNA BADULINA
National Research, Tomsk State University, Lenin Avenue, 36, Tomsk, 634050, Russia
LARISA NIKOLAEVNA ZIBAREVA
National Research, Tomsk State University, Lenin Avenue, 36, Tomsk, 634050, Russia
OLGA VICTOROVNA KOVZUNOVA
SSI Central Botanical Garden NAS of Belarus, Surganova St., 2c, Minsk, 220012, Republic of Belarus
*Author to whom correspondence should be addressed.
Abstract
The hairy root cultures of the ecdysteroid-containing species Silene linicola were obtained. The resulting cultures were analyzed for ecdysteroid content by means of high-performance liquid chromatography. A number of nonpolar and mid-polar ecdysteroids were detected. It was demonstrated that the presence of sodium acetate (150 mg l-1) in the nutrient medium promotes biosynthesis of a more diverse set of ecdysteroid compounds. Estimation of the levels of the major ecdysteroid 20-hydroxyecdysone showed that its general content in all samples varies within the range 0.13-0.18%. The results provide evidence that the use of hairy root cultures of S. linicola allows one to obtain a stable yield of the high levels of phytoecdysteroids and to enhance the level of biosynthesis using precursors of biosynthesis.
Keywords: Silene linicola, 20-Hydroxyecdysone, hairy roots, sodium acetate, growth parameters