ANTIOXIDANT STUDIES OF ONE HERBAL FORMULATION, KUTAJARISHTAM
MUTTEVI HAYAGREEVA KUMAR
Department of Anatomy, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chromepet, Chennai, India.
D. SHARMILA
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, India.
K. PRABHU
Department of Anatomy, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chromepet, Chennai, India.
MUDIGANTI RAM KRISHNA RAO *
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, India.
G. BHUPESH
Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chromepet, Chennai, India.
S. VASANTH
Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chromepet, Chennai, India.
SHRUTHI DINAKAR
Kottakal Arya Vaidya Sala, Chennai, India.
B. DEEPALAKSHMI
Department of Zoology, Ethiraj College for Women, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
The present study measures the three antioxidant assays, namely, ABTS, DPPH and FRAP by standard protocols for one ayurvedic medicine, which is used to treat diseases like to treat weak digestion, vomiting, diarrhoea, indigestion, dysentery, irritable bowel syndrome etc. The IC50 value for ABTS radical scavenging effect of Kutajarishtam was found to < 0.156 µg/ml and the standard drug, Ascorbic acid was 208.87 µg/ml. The IC50 value for DPPH radical scavenging assay of Kutajarishtam was found to be 1.968 µg/ml and the standard drug, Ascorbic acid was 8.158 µg/ml. The concentration of standard, ascorbic acid was taken as 5, 10, 20, 40, 80, 160 and 320 µg/ml while concentration of sample, Kutajarishtam was taken as 0.156, 0.3125, 0.625, 1.25 and 2.5 µg/ml. This variation in concentration was undertaken to have a clear understanding of its antioxidant property. The results indicate very good antioxidant activities of Kutajarishtam and this could be one of its important medicinal mechanisms.
Keywords: Antioxidant, ABTS, DPPH, FRAP, Kutajarishtam