GENETIC STUDY FOR PHLOGENY IN ALGAE USING RFLP
R. KAMALAMBIGESWARI *
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai-73, India.
L. JEYANTHI REBECCA
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai-73, India.
E. KOWSALYA
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai-73, India.
S. SHARMILA
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai-73, India.
D. SHARMILA
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai-73, India.
*Author to whom correspondence should be addressed.
Abstract
Tamil Nadu has many coastal regions since it’s the tip touching the Indian Ocean. There are many different coasts where seaweeds and algae are easily obtained. Two different coasts were selected and the species were collected. They were identified. After identification, the DNA was isolated and restricted with different restriction enzymes, then electrophoresed. Using labeled molecular marker, number of polymorphism was counted in case of restriction fragment length polymorphism. Using the data from RFLP, molecular maps for each species were constructed. The results revealed that there was a diversity and variability among the same species of organisms obtained from different locations.
Keywords: Sargassum sp., Enteromorpha sp., RFLP