DETERMINATION OF LEVEL OF SEED INFECTIVITY CAUSED BY RICE (Oryza sativa) SEED BORNE MYCOFLORA: AN in-vitro STUDY
TANMAY GHOSH *
Department of Biotechnology, NIT Durgapur, Burdwan, W.B., India.
M. K. BISWAS
Department of Plant Protection, P. S. B., Visva-Bharati, Sriniketan, W. B. 731236, India.
KAUSTAV AIKAT
Department of Biotechnology, NIT Durgapur, Burdwan, W.B., India.
*Author to whom correspondence should be addressed.
Abstract
The study aimed to isolate and determine seed-borne fungi associated with Cereal crop- Rice .The seeds sample were procured from Burdwan, W.B, India. The seed sample was hold on in artifact bag. Total 5 used to were wont to identification of seed borne fungi. The four flora genera, Rhizopus, Fusarium, Alternaria, Rhizoctonia, Cochlioboplus was found to be conspicuously related to totally different seeds of rice. Among them the share of incidence of Rhizopus sp. is predominently high all methods, used in isolation. The 18S rRNA sequence based molecuar methodology performed for phylogenetically determine the foremost pathogenic Rhizopus oryzae. Histopathology of apparently in infected seeds confined to plant structure fragments by Rhizopus oryzae in episperm and reproductive structure, embryonic region of Rice.
Keywords: Seed infectivity, seed borne mycoflora, Rhizopus oryzae, phylogenetic identification, histopathological study.