PESTICIDE INDUCED GENOTOXICITY ASSESSMENT IN MUNG BEANS BY DNA FRAGMENTATION ASSAY
HARISH CHANDRASEKHAR *
Center of Excellence for Microfluidics and Diagnostics, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
P. B. RAMESH BABU
Center of Excellence for Microfluidics and Diagnostics, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The current experiment was undertaken to determine and compare the breakage in plant DNA of Vigna radiate (mung bean). The mung bean was grown in two insecticides one is chloropyrifos and another one is a soft drink. Method followed to determine the DNA breakage is DNA fragmentation assay. Viable counting was estimated for each plant and fully enhanced and brought up for a study of statistic measure of which had growth issue and how many seeds didn’t accept the chemical and took it in an account for a reasons. Farmers in India have been using soft drinks as a cheaper alternative to pesticides. So here I am treating my plant with soft drink also to see whether it will cause DNA breakage. After completing DNA fragmentation assay we came to know that the control plant shows a good set of smear and darker DNA but in other plants pesticide has a sight faded smear and DNA and pesticide has a faded DNA. The aim of this study is to investigate the connection between occupational exposure to various pesticides and harm to DNA.
Keywords: Vigna radiate, mung bean, pesticides, DNA fragmentation assay.