DNA CONTENT, MORPHOLOGICAL AND MOLECULAR MARKER ANALYSIS FOR THE EVALUATION OF NEW MANDARIN HYBRIDS

Main Article Content

MAJDA STITOU
OUIAM CHETTO
ABDELHAK TALHA
NAJAT HANDAJI
YASSMINA ELBAHLOUL
RACHID BENKIRANE
HAMID BENYAHIA

Abstract

The objective of this work was to characterize the new mandarin hybrids by morphological and molecular analysis. Forty hybrids from two crossing between Afourer mandarin and Nova mandarin were evaluated by morphological traits of leaves, cytological and by microsatellite markers SSR. The morphological characteristics suggested that almost all hybrids showed difference statistically highly significant (P < 0.01) for leaf length, leaf width, and ratio leaf lamina length/width. The results of cytometry analysis exhibited that all the hybrids are diploid. Molecularly, one of the four primers detected polymorphisms and three hybrids were selected. This study provided a microsatellite based approach for parentage assignment in hybrids, and that will be useful for investigation of genetic background and selection of new citrus species and varieties.

Keywords:
Citrus, Mandarin hybrids, morphological analysis, cytometry, molecular analysis, microsatellite markers SSR.

Article Details

How to Cite
STITOU, M., CHETTO, O., TALHA, A., HANDAJI, N., ELBAHLOUL, Y., BENKIRANE, R., & BENYAHIA, H. (2020). DNA CONTENT, MORPHOLOGICAL AND MOLECULAR MARKER ANALYSIS FOR THE EVALUATION OF NEW MANDARIN HYBRIDS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(1-2), 39-48. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/4922
Section
Original Research Article

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