LOW-COST MEDIA IS AN ALTERNATIVE NOVEL TECHNIQUE TO REGENERATE in vitro Gynura procumbens (Lour.) Merr. INIMITABLE MEDICINAL PLANT

Main Article Content

MD. RASAL- MONIR
MD. NASIR HOSSAIN SANI
JASIM UDDAIN
SOURAV MODAK
SAMAR BISWAS
MOHAMMAD HUMAYUN KABIR

Abstract

The present research was carried out to develop an alternative in vitro low-cost culture medium for regeneration of an inimitable medicinal plant Gynura procumbens. The nodal segment has been utilized as explant and six separate types of low-cost medium (LCM) which includes LCM-1 (100 mL coconut water); LCM-2 (150 mL coconut water); LCM-3 (200 mL coconut water); LCM-4 (100 mL banana pulp); LCM-5 (150 mL banana pulp); LCM-6, (200 mL banana pulp) and the complete strength MS medium was employed as a control treatment for the standardization of in vitro low-cost regeneration protocol for the Gynura procumbens. All the culture media were supplemented with 2.0 mg / L BA + 0.5 mg / L NAA for shooting and rooting with 0.5 mg / L IBA. Compared to other low-cost culture media, culture media LCM-2 showed the best results in shoot initiation, shoot multiplication, and root formation. As regards cost analysis, LCM-2 appears to minimize cost (78.22%) of the medium per plantlet compared to the regular standard MS medium. Medium of acclimatization consisting of garden soil + Vermicompost + coconut dust (1:1:1, v / v) demonstrated the best hardening medium with maximum survival rate and robust growth performance. Our findings suggest that the LCM-2 culture medium for the regeneration of Gynura procumbens aseptically is an economically cost-efficient protocol and an alternative to the regular standard MS medium.

Keywords:
Gynura, medicinal plant, low-cost medium, tissue culture, regeneration

Article Details

How to Cite
MONIR, M. R.-, SANI, M. N. H., UDDAIN, J., MODAK, S., BISWAS, S., & KABIR, M. H. (2020). LOW-COST MEDIA IS AN ALTERNATIVE NOVEL TECHNIQUE TO REGENERATE in vitro Gynura procumbens (Lour.) Merr. INIMITABLE MEDICINAL PLANT. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(15-16), 12-22. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5119
Section
Original Research Article

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