RAPID In vitro PROPAGATION AND GENETIC FIDELITY EVALUATION OF MEDICINALLY IMPORTANT MEXICAN CORIANDER (Eryngium foetidum L.)

Main Article Content

BISWAJIT JENA
BHAGYASHREE BISWAL
ALOK KUMAR GIRI
REENA PARIDA
LAXMIKANTA ACHARYA

Abstract

Eryngium foetidum L., a biennial herb has been used tremendously as a medicinal plant mostly in tropical regions of the world. With its wide area of ethno-medicinal uses and the growing sector of ayurvedic medicine have increased demand for the plant. Hence there is an essential need for conservation and cultivation of this plant for the future. Here, an efficient large-scale clonal propagation protocol has been designed using the peduncular part derived from greenhouse-maintained plants. Murashige and Skoog’s (MS) media in addition with 3.0 mg l-1 N6-benzyladenine (BA) and 1.0 mg l-1 Indole-3-acetic acid (IAA) was found optimum for shoot proliferation (11.3±0.64 shoots per explant) and root development (24±0.40 roots per shoot) Successive plantlet cultures were established by repeated sub-culturing on a fresh medium after each yield of newly formed shoots. The plantlets were successfully transferred to the soil after acclimatization. The plants have been maintained in field condition for three years. Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeats (ISSR) were used for validating the genetic fidelity of the in vitro generated plants at every four month interval for three years and after analysis, revealing a homogeneous amplification profile for all in vitro plants analysed against the mother plant.

Keywords:
Eryngium, RAPD, ISSR, in vitro regeneration, genetic fidelity.

Article Details

How to Cite
JENA, B., BISWAL, B., GIRI, A. K., PARIDA, R., & ACHARYA, L. (2020). RAPID In vitro PROPAGATION AND GENETIC FIDELITY EVALUATION OF MEDICINALLY IMPORTANT MEXICAN CORIANDER (Eryngium foetidum L.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(15-16), 79-88. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5153
Section
Original Research Article

References

Duke JA. Duke's handbook of medicinal plants of Latin America, USA: CRC Press. Taylor and Francis. 2009;298–300.

Kumar V, Datt B. Eryngium foetidum L.: An overlooked species for erstwhile Madhya Pradesh. J Econ Tax Bot. 2006;30: 303–304.

Paul JHA, Seaforth CE, Tikasingh T. Eryngium foetidum L.: A review. Fitoterapia. 2011;82(3):302-308.

Standley PC, Williams LO. Flora of Guatemala – Myrsinaceae. USA: Chicago Natural History Museum, Part VIII 1 and 2. 1966;24:46-47.

Forgacs P, Jacquemin H, Moretti C, Provost J, Touche A. Etudes phytochimiques et activities biologiques de 18 plantes de la Guyane Francaise. Plant Med Phytotherapie. 1983;17:22–32.

Anam EM. A novel triterpenoid saponin from Eryngium foetidum. Indian J Chem Sec B. 2002;41B:1500–1503.

Arbain D, Cannon JS, Kartawinata KA, Djamal R, Bustari A, et al. Survey of some West Sumatran Plants for alkaloids. Econ Bot. 1989;43:73–78.

Simon OR, Singh N. Demonstration of anticonvulsant properties of an aqueous extract of Spirit Weed (Eryngium foetidum L.). West Indian Med J. 1986;35(2): 121-125.

Garcia MD, Saenz MT, Gomez MA, Fernandez MA. Topical anti-inflammatory activity of phytosterols isolated from Eryngium foetidum on chronic and acute animal models. Phytother Res. 1999;13: 78–80.

Arokiasamy S, Ignacimuthu S. Plant regeneration from mature leaves and roots of Eryngium foetidum L. a food flavouring agent, Curr Sci. 1998;75:664-666.

Yasseen YM. In vitro regeneration, flower and plant formation from petiolar and nodal explants of Culantro (Eryngium foetidum L.), Society in vitro Biol. 2002;423-426.

Gayatri MC, Madhu M, Kavyashree R, Dhananjaya SP. A protocol for in vitro regeneration of Eryngium foetidum L. Indian Journal of Biotechnology. 2005;5: 249-251.

Larkin P, Scowcroft W. Somaclonal variation-A novel source of variability from cell cultures for plant improvement. Theor Appl Genet. 1981;60:197–214.

Lakshmanan V, Venkataramareddy SR, Neelwarne B. Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers. Electron J Biotechnol. 2007;10: 106–113.

Anandan R, Prakash M, Deenadhayalan T, Nivetha R, Kumar NS. Efficient in vitro plant regeneration from cotyledon-derived callus cultures of sesame (Sesamum indicum L.) and genetic analysis of True-to-Type regenerants using RAPD and SSR markers, South African Journal of Botany. 2018;119:244-251.

Ntuli NR, Tongoona PB, Zobolo AM. Genetic diversity in Cucurbita pepo landraces revealed by RAPD and SSR markers. Scientia Horticulturae. 2015;189: 192-200.

Murashige T, Skoog F A. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-497.

Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13–15.

Acharya L, Panda PC. Validation of generic status of different taxa in the subtribe Cassiinae (Leguminosae: Caesalpinoidae) using RAPD, ISSR and AFLP markers. International Journal of Plant Physiology and Biochemistry. 2010;2(2):18-28.

Martin KP. Organogenesis on root, leaf, stem-disc and scape explants of Eryngium foetidum L., a rare medicinal plant. J Herbs Spices Med Plants. 2005;11:9–17.

Sudarshana MS, Akshatha BH, Mahendra C, Guru C. In vitro somatic embryogenesis in Eryngium foetidum L. - a medicinal herb. International Journal of Pharma and Bio Sciences. 2014;5(1):103-111.

Chandrika R, Vyshali P, Saraswathi KJT, Kaliwal BB. Rapid multiplication of mature flowering plant of Eryngium foetidum L. by in vitro Technique. International Journal of Biotechnology Applications. 2011;3(4): 114-117.

Chandra S, Bandopadhyay R, Kumar V, Chandra R. Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnol Lett. 2010;32:1199.

Ray T, Dutta I, Saha P, Das S, Roy SC. Genetic stability of three economically important micropropagated banana (Musa spp.) cultivars of lower Indo-Gangetic plains, as assessed by RAPD and ISSR markers. Plant Cell Tiss Org. 2006;85(1): 11–21.

Bornet B, Branchard M. Nonanchored inter simple sequence repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting, Plant Mol Biol Rep. 2001; 19(3):209–215.

Peredo EL, Revilla MA, García RA. Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of Organogenic calli. J Plant Physiol. 2005;163(10):1071–1079.

Palombi MA, Damiano C. Comparison between RAPD and SSR molecular markers in detecting variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep. 2002;20:1061–1066.

Mallón R, Rodríguez-Oubiña J, González ML. In vitro propagation of the endangered plant Centaurea ultreiae: Assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell Tiss Organ Cult. 2010;101:31–39.