ISOLATION AND MOLECULAR IDENTIFICATION OF A NOVEL Fusarium oxysporum FROM SUMAC PLANT
ASMAA MANSOUR AL- HAKEEM
Department of Microbiology, College of Science, AL-Karkh University of Science, Iraq.
HUDA HAZIM AL- TAEE
Department of Plant Protection, College of Agriculture and Forestry, University of Mosul, Iraq.
MOHAMMAD J. AL- JASSANI *
Department of Microbiology, College of Science, AL-Karkh University of Science, Iraq.
*Author to whom correspondence should be addressed.
Abstract
Sumacs Rhus coriaria L. has long been used as food spice, food colorant and preservative, and it is also used in cosmetics, medicines and in the animal leather industry.
Fungi infecting this plant were isolated and identified by morphological and molecular methods, particularly for the most dominant isolates by PCR and sequencing the ITS region.
The isolation results showed the presence of Fusarium oxysporum with the highest frequency of 27% followed by Cylindrocarpon, Fusarium solani, Rhizoctonia solani and Alternaria sp. with frequency rates of 23%, 20%, 16% and 10%, respectively, while the Verticillium dahlia and Cephalosprum sp. were the least frequent with a rate of 2% for both.
Sequencing revealed new Fusarium oxysporum isolates that have been registered at the GeneBank database.
Keywords: Fusarium oxysporum, Rhus coriaria, PCR, sequencing